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Tag Archives: writing
Tuesday 06/26/12
10:30 A -5:00P, 9:00P – 11:30P in situs day 2 short fix 2-14 hour yw and Pc embryos. rehydrate and stain 2-14 hour short fix embryos with Su(Z)12. Pc-GFP embryos also stained with ch a-GFP. vball lost. Synthesized Bac 1 … Continue reading
Posted in Summaries
Tagged embryo collection, embryo labeling, in situs, probe making, STORM analysis, writing
Comments Off on Tuesday 06/26/12
Monday 06/25/12
9:45 A – 7:00P, 9:30P – 11:45P Need new collection cages FISH + K27 on O/N embryos day 1 Flipped 3x Mat Gal4 cages. Running Z-calibration analysis on Suz647 data (with Quadview) Collecting new z-cal bead data in 512 and … Continue reading
Sunday 06/24/12
Feedback from ML on dynamics paper. Need to chose journal. Homogeniety section could use some tightening.
Monday 06/18/12
working on snail bistability manuscript Comparison of snail patterning to optimal decision making theory for two alternative forced choice (see Holmes et al), see post.
Tuesday 05/29/12
1:00 P – 4:00P, 9:00P-12:00A running insight analysis on K4 K27 data (11:00A) using only single iteration for computation of z-calibration curve. compare structure of K4, K27me3 and K27ac chromatin (?) troubleshooting issue with non-convergence of z-calibration bead data by … Continue reading
Monday 04/09/12
10:00 A – 10:30 P Lab meeting (Doory) Fix alignment of 750 laser with Colenso’s help Freeze Bcd-GFP and eve-GFP bottles. NuGru presentation (Andi) Working on snail manuscript supplemental figure captions Download ModEncode data for H3K4me3 and H3K27ac Etch and … Continue reading
Sunday 04/08/12
4:30 P – 8:30 P 4x snail data looks excellent. See post. working on snail manuscript supplement
Friday 03/30/12
9:20A — 8:00 P Shuttle to Med campus to see Xiaochen talk (White lab student interviewing for Perrimon / Bulyk labs) Very clean CTCF data. Should try this antibody in STORM Finalize ACS application. Submitted paper copy and FEDEX’d. Still … Continue reading
Thursday 03/29/12
10:15 A – 8:00P Working on snail dynamics paper. Developing new introduction. new script to change Fig 6 to color code nuclei by mRNA count class rather than direct mRNA levels — easier to interpret with nuclear channel added in.
Wednesday 03/28/12
10:15 A – 8:00P Analyzing Psc and Pho data with new alignment routine (summary results still need to add images to lab notebook, see protected post) Working on snail manuscript