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Tag Archives: fly work
Thursday 02/16/2012
9:40 A — 12:10 A collect virgins O/N STORM run did not finish on schedule (68 of 112 images done) contact Wu lab about choice DNA FISH regions install GTX580 card. install latest NVIDIA drivers received Rad21 antibodies in 50% … Continue reading
Wednesday 02/15/12
9:45 A — 11:00P Etching Dm0-1 slides (25-30 min) for STORM. (Slide 69 in records) Flip fly cage, 10A Washing out primary antibody in PcG stained embryos in situs for PcG stained embryos: hox and en locus (see records) organized … Continue reading
Sunday 02/12/12
9:45 A — 5:20P, 8:30P — 11:50P Finish STORM O/N of S45 (13-introns) setup STORM run of Pc Flip fly cage (10:50P) Flip fly cage (1:50P) Hal holds control of 647 laser AOTF against labview. Fixed. Analyzing STORM data by … Continue reading
Posted in Summaries
Tagged confocal, fly work, presentation, STORM, STORM analysis
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Friday 02/03/12
10:10 A – 9:30 P Flip collection cages, 10:15 A Prep new coverslips (2 racks) Flip collection cages 12:15 P clear sna[D] flies for virgin collection Flip fly stocks Flip MP09 and 4x snail flies transfer laid embryos to make … Continue reading
Posted in Fly Work, Summaries
Tagged fly work, presentations, sectioning
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Tuesday 01/31/12
10:00 A — 12:55 A Flip fly cages (MP08 and yw) Review JCC app. Submit JCC app. Working on snail paper final results/model section. Syncing datafiles on snail patterning sectioning: Cut 2 slides of s04(13-introns), one of slide 1, one of … Continue reading
Monday 01/30/12
10:00 A – 8:00 P Projecting snail data (MP08_20x) Fixing minor issues in JCC app from N. Francis suggestions uploading and formatting JCC app should have submitted university letter sooner, Matt helps solve. analyzing MP08 rescue data. 20x images not … Continue reading
Tuesday 01/24/12
10:00A – 1:00 A Finish v5 of JCC draft, sent to CB and DB. New version of figure for JCC app. 2 new sna[D] bottles for virgin collection to remake MP12 rescue line. DB MP12 flies flipped back to vial … Continue reading
Posted in Summaries
Tagged communication, confocal, fly work, staining
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Tuesday 11/22/11
10:00 A – 8:00P, 9:00P-10:00P Finishing annotation of revised CheckDotUpDown code to do 3D dotfinding. Running test analysis of MP06 data. New method for finding unique spheres is easier to read but much much slower, need to find some solution … Continue reading