Tag Archives: cloning

Monday 03/19/12

Posted on March 19, 2012 by admin

9:40A – 11:55 P Lab meeting presentation: Jeff Moffit 1 hr RT O/N primaries washing out O/N primary antibody labels ACS draft app to Matt Fly meeting receipts organized, sent to Matt Promoter synchrony paper feedback for Mounia and Jacques … Continue reading →

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Sunday 03/18/12

Posted on March 18, 2012 by admin

11:00 A – 8:45 P No colonies on plates Finish O/N STORM run.  Run failed at movie 121, unknown error message. attempt bead feducial drift correction: polysterene beads good autofluoresnce in all but 750, but bleach fast. 405 beads not … Continue reading →

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Saturday 03/17/12

Posted on March 17, 2012 by admin

11:00 A – 10:00P Experimenting with heat fixing embryos working on ACS application filter extract new tracer beads.  Initial test suggests still contains raw dyes.  Tried spin. No obvious colonies on plates.  Back at 37C O/N. STORM imaging of Pho … Continue reading →

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Friday 03/16/12

Posted on March 16, 2012 by admin

9:00 A — 9:30P Finish O/N confocal run, save data Projecting data in Matlab Make new and autoclave: 2x YT aliquot IPTG and X-Gal Transform and plate competent cells, 5:30 plates at 37C O/N.  300 uL per plate PCR with … Continue reading →

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