Tag Archives: confocal

Friday 11/30/12

Posted on November 30, 2012 by admin

9:30A – 10:15 P Fly Work Collect sna[18] x Double balancer virgin and male F1s PM virgin collection STORM2 time. Colenso removing gas laser from STORM2 Mount ultra cryo section embryo for imaging sections not flat or hard to find. … Continue reading →

Posted in Summaries | Tagged , , , , | Comments Off on Friday 11/30/12

Wednesday 11/14/12

Posted on November 14, 2012 by admin

9:00A – 7:30P, 8:15P – 11:15P Prepping gel (low on Na Borax, and TAE making up new TAE) Test gel: YW postive control AbdB looks great.  YW negative control sim[D] is clean.  Improvised gel extractions not looking good. Try sim[D] … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Wednesday 11/14/12

Saturday 11/10/12

Posted on November 10, 2012 by admin

10:45 A – 8:00P Snail project stuff Collect virgin Pr/Tm3Z, Sp/CyOZ, and TM2/TM6 (males + virgins) Order primers for screening E(spl) and sim[D].  Also for en and inv introns DNA FISH probe design Wrote new matlab script for concatinating text … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , | Comments Off on Saturday 11/10/12

Sunday 11/04/12

Posted on November 4, 2012 by admin

11:00 A – 8:00P Cell culture and cell labeling Start washing out direct-labeled primaries from cell-culture plates. Split cells, toss old plates. plate cells on round cover glass in 12 well pates.  See if these adhere any better for FISH-on … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Sunday 11/04/12

Thursday 11/01/12

Posted on November 1, 2012 by admin

9:30 A – 7:30P, 8:30P- 12:00A Software install on STORM2.  Hal can’t detect drivers for national instruments panel. Overview of software install: see post Fly work Collect virgins Sort and cross Espl/sim flies Clear MTDs into 2 new bottles, move … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Thursday 11/01/12

Wednesday 10/31/12

Posted on October 31, 2012 by admin

9:45 A – 8:40P, 9:45P-11:45P Submit revised snail kinetics paper. Collect virgin Espl/sim cross to TM2/TM6 Collect virgin Act5 Gal4.  Cleared and added new bottle of these to virign collection. washout Pc antibody from O/N MTD post fix 5 min … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Wednesday 10/31/12

Sunday 10/28/12

Posted on October 28, 2012 by admin

10:30 A – 1:45P, 4:45 – 8:15P Fly Work 10:30A collect virgin Espl/sim 12:00 P, flip collection cages 7:30 P, fix embryos, 0.5 – 7 hrs MTDs, Psc 1, and Pc 3.  Left on shaker.  Good embryos should be sunk … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Sunday 10/28/12

Saturday 10/27/12

Posted on October 27, 2012 by admin

10:30A – 8:ooP Stop O/N confocal by 11A. Confocal data check: Late positions start too low.  Not sure why — maybe middle position to close to edge changed microscope positions? Fly Work Collect virgin Espl[D]/ sim[D].   Flip fly stocks … Continue reading →

Posted in Summaries | Tagged , , , , | Comments Off on Saturday 10/27/12

Friday 10/26/12

Posted on October 26, 2012 by admin

10:20 A – 7:30PM Fly Work  Set up collection cages for TRiP knockdown experiments: MTD x Psc 1 MTD x Pc 3 Set up new collection cage of MTD controls.  Pc labels looked weak and Pc in situs did not … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Friday 10/26/12

Tuesday 09/11/12

Posted on September 10, 2012 by admin

9:10 AM – 7:15 P, 9:00P – 11:55 P Log off Tuck (Reserved for Yari) – 7 Processes all doing final fit on ~500,000 molecule list (about 1 molecule/per sec!  These are gonna have to get killed…) Finish O/N STORM … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Tuesday 09/11/12