Tag Archives: fly work

Wednesday 11/27/12

Posted on November 28, 2012 by admin

10:15 A — 11:15 PM Polytene cell staining  hot washes in 2x SSCT (45 C in coplin jar in dry oven) check staining — cent probe looks good (rather small foci though) Antibody staining H3K9 did not work.  Should have … Continue reading →

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Tuesday, 11/27/12

Posted on November 27, 2012 by admin

9:40 A – 8:00P, 9:15P — 11:40P Fly Work collect virgin 2x HbZ. clear MTD x Pc1 parental generation. check Espl x sim crosses write to Maria to cancel Wed sectioning test gel PCRs No substantial bands New gel: Espl-DNA … Continue reading →

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Monday 11/26/12

Posted on November 26, 2012 by admin

9:35 A – 7:00P, 8:20P – 11:50P STORM chromewarp 3D has much more trouble vertically matching beads when we take the same field for each z.  Need to change this.    1 manual edit of the beadrun.xml file ought to … Continue reading →

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Wednesday 11/22/12

Posted on November 22, 2012 by admin

9:10 A — 8:00P, 10:30P – 12:45 A UltaCryo collect prepped samples 10A – 2P, cutting ultra cryo sections of 1-10hr MTD DFISH-fixed embryos For next time: denser embryo packing in smaller gelatin sections Cryo protect at least 30 min … Continue reading →

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Thursday 11/23/12

Posted on November 21, 2012 by admin

10:30 A – 7:30 P Thanksgiving Fly work expand YWs Flip stocks check crosses.  double HbZ not yet emerging.  recombineering crosses looking bleak, we’ll see in anotehr 5-6 days… Probe making New library stats spin down dissolving probe.  Gently Pippett … Continue reading →

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Thursday 11/15/12

Posted on November 15, 2012 by admin

9:15 A — 6:45 P, 8:30P – 12:00A collect virgins for sim Espl[D] recombineering new cross, sna males x Sp/CyoZ; TM2/TM6 virgins clear MTDs and TM2/TM6s for virgin collection (peak emergence for latest bottles?) pulverize and refreeze Espl[D] and sim[D] … Continue reading →

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Friday 11/09/12

Posted on November 9, 2012 by admin

9:30 A – 8:30P Making double mutants Collect virgin TM2/TM6 flies Clear Pr/TM3,hbZ and Sp/CyOZ for virgin collection to remake double balanced 2,3 flies. TM6b = AntpHue1 . TM2 = Ubx130esemc2 . E(spl) and sim[D](7646) both have TM6b, Tb.  Should be lethal with TM6. … Continue reading →

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Friday 11/02/12

Posted on November 2, 2012 by admin

9:30 A – 7:48 PM, 10:50 – 11:45PM Fly work Flip collection plates on MTD x SCM, MTD, and sim[D], 9:30A Flip collection plates, sim[D] 1:15  P Collect virgin Act5-Gal4, MTDs, and sim/Espl.  Sort sim/Espl against humoral bristles. New crosses … Continue reading →

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Tuesday 10/30/12

Posted on October 30, 2012 by admin

9:30 A – 7:30P, 8:30P – 11:50 P Flies Flip collection plates. # Save MTDs to plate into new bottles.  All in one, need high density. Collect virgin Espl/sim, cross to TM2/TM6 males. Collect virgin Act5-gal4 Make new AJ plates … Continue reading →

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Monday 10/29/12

Posted on October 29, 2012 by admin

10:40 A – 8:15 P lab meeting canceled due to hurricane Sandy contact ML about revisions. Got suggestions for graphical abstract, now implemented.  Waiting for response on text. contact Ting about T7 primers Plan to meet with Brian and Fred (Thursday? … Continue reading →

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