Tag Archives: mRNA counting

Monday 06/27/11

Posted on June 27, 2011 by admin

10:00 A — 6:45 P, 7:30P – 9:20 P Discussion with Mounia and Jacques from Gordon Conference Discussion with ML from Gordon Conference observations.  Set up priorities. Progress Report. Errors in data analysis for Espl, vnd double null embryo analysis. … Continue reading →

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Protected: snail y Hz quantification

Posted on June 27, 2011 by admin

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Progress Report 06/27/11

Posted on June 27, 2011 by admin

Progress Report Top Findings: 1. Effect of bcd does on hb expression: 6x bcd have peak hb levels similar to 2x bcd, 1x bcd are close but a little less hb and more variable between embryos. 2. hb-BAC minus shadow … Continue reading →

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Protected: MP09 intensity sensitivity

Posted on June 23, 2011 by admin

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Wednesday 06/22/11

Posted on June 22, 2011 by admin

9:40 A -3:30 p Finish ON confocal.  Data acquisition looks good, 10 embryos finished. Rerunning unsupervised dot counting with higher threshold to reduce noise in y channel on MP09_c.  (matched .05 filter rather than .02). Layout for lab-meeting.

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Protected: bcd 1x v 6x cont

Posted on June 21, 2011 by admin

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Protected: bcd 1x and 6x counting

Posted on June 20, 2011 by admin

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Monday 06/20/11

Posted on June 20, 2011 by admin

10:00 A — 7:00P, 8:00P – 10:00P Mount slides Confocal, MP07 hets Test samples (s02, some background, weak en in test sample, need more imaging) Biophysics webpage development: added academics page and random image widget. Analyzing sna[18] MP10 data Reading … Continue reading →

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Sunday 06/19/11

Posted on June 19, 2011 by admin

3:45P – 7:20P, 8:10 P — in situs day 3 used old block on samples, then rinsed and switched to new block — don’t store diluted sample. Fixed page specific posts for biophysics page using widget logic (widget context doesn’t … Continue reading →

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Protected: Analyze snail shadow deletion counts

Posted on June 19, 2011 by admin

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