Tag Archives: Ph

Wednesday 10/30/13

Posted on October 30, 2013 by admin

9:00a – 10:50p STORM Finish O/N E10 imaging Hao imaging set up O/N imaging of E12 (600 kb G) with 20:30,000 bead dilution. Qualitative observations: some G regions quite large, seem pretty decompact. Others more compact? Laser power dropped to … Continue reading →

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Ph Project To Do

Posted on October 30, 2013 by admin

Goals: Counts co-localized vs. not co-localized in Mutant vs Wildtype Psc knockdown and imaging More numbers for Ph in Ph-Wt and Ph in Ph-M Modeling: a. get correct random walk behavior from polymer model b. reproduce data trend with ka … Continue reading →

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Sunday 10/20/13

Posted on October 19, 2013 by admin

1:00p-2:30p, 5:00p-11:50p Goals: Today write up some notes on Stowers presentations. layout slides for Rowland Center talk (in progress) This Week Write up Banff Report! Probe Making PCR: Large Regions (primer concentration) D11 100 kb percicentric Green (320 ng/uL) D12 … Continue reading →

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Friday 10/11/13

Posted on October 11, 2013 by admin

10:00a – 10:30p Goals send Ph modeling results to Ajaz work on presentation send reminder about racing Saturday STORM analysis Copying F11 data to ProBox2. Delete data from Storm2 Running splitdax on no405Primary, E07fail, F09, and F01v2 (latter 2 including … Continue reading →

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Protected: Ph Meeting Notes

Posted on October 11, 2013 by admin

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Friday 08/23/13

Posted on August 23, 2013 by admin

10:15a – 8:10p, 8:50p-11:05p BX-C tiling Finish O/N STORM of Ubx spots start 65C 50% formamide rinse out of probe (30 min) Hot wash of newly labeled Ubx (orginally AbdA): 60C 2x SSCT Image Ubx. Correspondence of cells and correspondence … Continue reading →

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Wednesday 08/21/13

Posted on August 21, 2013 by admin

9:45a – 11:30p cell staining heating buffer. wrong buffer, no formamide in 60C hot wash. Did I use the formamide buffer yesterday? I recall heating a 50mL tube and I don’t think I have 2x SSCT in a 50mL tube… … Continue reading →

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Monday 07/22/13

Posted on July 22, 2013 by admin

9:30 A – 7:00 P, 8:30 P – 11:50 P STORM Finish O/N set up new STORM, coverslip 7. high background, must by cy5-primary. not too much switching, maybe single labeled cy5 primary. Responsive to 405 but only at substantial … Continue reading →

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Wednesday 07/10/13

Posted on July 10, 2013 by admin

10:00 A – 7:40 P, 8:30 – 10:00 P General laboratory safety training refresher check HoxD server jobs: still heavily backed up check STORM analysis / drift correction: Y complete. Others still running. update notebook entries from yesterday. Review working … Continue reading →

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Monday 06/24/13

Posted on June 24, 2013 by admin

10:30 A – 6:30 P, 8:30 P – 10:45 P General post-conference paperwork Ph project working on explanation of clustering quantification fig send fig to Ajaz. plan to do Pc or Psc later this week. see post Planning sign up … Continue reading →

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