Tag Archives: STORM analysis

Sunday 01/13/13

3:30P – 11:55 P Fly work Flipped most of rescue crosses.   Several vials extinct.  Should still have more vials than I want to screen though. New crosses of SCM males to MTD virgins (2 vials). Examine current crosses of … Continue reading

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Tuesday, 11/27/12

9:40 A – 8:00P, 9:15P — 11:40P Fly Work collect virgin 2x HbZ. clear MTD x Pc1 parental generation. check Espl x sim crosses write to Maria to cancel Wed sectioning test gel PCRs No substantial bands New gel: Espl-DNA … Continue reading

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Sunday 11/25/12

11:00 A – 5:00P, 8:00P – 10:00P Code development Working on new Matlab tool for rapid color channel adjustments to images and drag and drop image loading capabilities (faster and more convenient than doing color switching in photoshop). GUI is now in … Continue reading

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Monday 11/12/12

9:45 A – 7:30 P, 8:45P – 10:00P Troubleshooting issues with bead warping wrote short translation correction to (1D polywarp) to correct the gross aberrations introduced by the STORM4 quadview. Still seems to be a shift.  Might be due to … Continue reading

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Tuesday 10/16/12

9:00 A – 6:50P, 8:20P -11:15P discuss CMAKE minGW sys with JM 9:30 A flip collection plate Working on BioPhys J review. 11:30 A – check samples from oven substantial issues with resin quality: 1 sample R solid at 70C. … Continue reading

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Monday 10/15/12

9:30 A – 7:30 P Flip collection plate on MTD flies.  starting to lay…. sorta. looking at aptamers for histone labeling (see Mendeley). DNA-FISH + protein prep for STORM protocol test try 2:  samples now embedding. [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 302 sheet=8] … Continue reading

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Protected: Analysis of En locus k27me3

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Protected: Pc clustering over time (at SR)

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Monday 09/10/12

9:40 A – 5:00P, 8:15P – 11:00P Restart data run on Tuck Transfer files from STORM4 (en STORM from 9/6… oops). Etching slide S6 for imaging today. Move ND filter 5 from STORM2 750 line to STORM4. Colenso also notices … Continue reading

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Saturday 09/08/12

10:15 A – 7:00P, 9:00P-10:00P Working with new ways to plot snail data updated annotation on snail modeling and snail Pr/enh data plotting. modeling repressor strength. plotting summary of k27 STORM results at Hox loci (see post) working on plotting … Continue reading

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