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Protected: project 2 updates
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Posted in Research Planning
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Monday 06/05/13
9:30 A – 7:45 P, 8:45P – 11:55 P Oligopaints Running out gradient gels (one at a time) hotter is better with mis-priming. using 20 bp PPT primer and library specific NP primer: with adapters: Check Ph stains booked confocal … Continue reading
Sunday 05/05/13
STORM Analysis Fixed bug in RunDotFinder that was causing DaoSTORM analysis on to correctly overwrite/rerun analysis (DaoSTORM method check had method in quotes, causing failure). Project 2 Data analysis notes OligoPaints Running 2.5 mL of PCR using PPTC primer at … Continue reading
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Protected: project 2
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Posted in Microscopy, Research Planning
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Saturday 05/04/13
5:50 P – 8:30 P Sequential staining Attempting to align images from sequential staining. Correspondence not obvious. Substantial translation and rotation, need to fix these with feducials. Probe making Run column cleanup on LC libraries after 10 round PCR using … Continue reading
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Friday 05/03/13
10:00 A – 8:00 P, 9:00 P – 11:00 P Joint project Help Hao relocate cells from yesterday and check sequential hybe Succesfully relocated all cells. Substantial Cy3 signal still present, but much more Atto647N. Need to analyze images. STORM … Continue reading
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Thursday 05/02/13
10:30 A General stuff reply to Olivia about quantifying nuclear concentration, mRNA FISH Need to fedex keys and forms back to U Chicago. Wrote to Matt to get acct info # Enter all accepted Banff Invites into BIRS system, including … Continue reading
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Saturday 04/27/13
10:30 A – 12:30P, 2:30P – 7:30P, 9:00P – 11:45P Other looking for high quality humidity meter for sectioning This looks promising. Need Pcard to be active again to purchase: http://www.amazon.com/Extech-445815-Humidity-Meter-Remote/dp/B000GFCN1I/ref=sr_1_8?ie=UTF8&qid=1367092332&sr=8-8&keywords=humidity+sensor Presentation Finished first draft through slides. Need to rehearse … Continue reading
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Friday 04/26/13
10:00 A – 9:00 P Shadow enhancer modeling worked out algorithm using binary codebook to compute the random order binding matrices for arbitrary number of binding sites. upgrading laptop to Ubuntu 13.04 Monet froze during scheduled (requested) windows reboot/install updates. … Continue reading
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Lezhei seminar
Solar cell construction atomic layer deposition of SnS (photon absorbing layer). add Sn-X, binds surface, add S2H, replaces X with S. Rpt to make 1500 nm layer Zn(O,S) layer — combination very sensitive effect on solar cell efficiency. Stats: Voc … Continue reading