Author Archives: admin

denaturing diagnostic gels

Posted on April 14, 2013 by admin

Gel 1, 5% Agarose, 200 V in sodium-Borax (300 V melts gel). Gel 2, 15% Urea, 120V 2 hrs Gel 3 15% Urea gel, 120 V 50 min in 37C room Gel 4, 10% precast Urea denaturing gel, 120 V, … Continue reading →

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Saturday 04/13/13

Posted on April 13, 2013 by admin

3:00 P – 8:00 P, 9:00 P – 11:00 P Working on review Finalize discussion Touch up figures Touch up figure captions reread once through Send to Stas, Peter, Carl and David for comments OligoPaint troubleshooting Qubit data 10% denaturing … Continue reading →

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qubit data

Posted on April 13, 2013 by admin

concentrations y1 D = 56.1 ug/mL (50*8 uL) = 22.4 ug y1 D F = 82.6 ug/mL (150 uL) = 12.3 ug y10 D = 294 ug/mL in 110*8 uL = 258 ug y10 D F = 96 ug/mL in … Continue reading →

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Reorganizing Protocols

Posted on April 12, 2013 by admin

Goals version managed protocols Protocols page directs to most recent version of the protocol Most recent version of protocol is linked to all previous revisions, and can be diff compared Day-to-day implementation of the protocol permalinks the version as it … Continue reading →

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qubit data

Posted on April 12, 2013 by admin

results prior to extraction y prot contr = too high BP 1/2 PCR = 38 ug/mL 7:20 P, reusing standards, 10.9 BP control = 72.4 ug/mL BP = 35 ug/mL Y-T em 1/2 = 9.16 ug/mL. 7:20P, reusing standards, = … Continue reading →

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Friday 04/12/13

Posted on April 12, 2013 by admin

Web Notebook Updates Switched to Markdown-on-Save plugin instead of WP markdown supports Markdown Plus features like tables etc. Saves original and markdown separately, no corrupting the original entry format because the markdown compiler doesn’t recognize things as a list. Reorganizing … Continue reading →

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Thursday 04/11/13

Posted on April 11, 2013 by admin

Oligo Paint Probe synthesis Test PCRs at 1 mL scale: Assymetric y-PCR, Y-adapter, no Y-L primer + 1uL of EtOH extracted Y PCR product at 85 ng/uL Assymetric b-PCR, B-adapter + 1 uL of EtOH extracted B PCR product at … Continue reading →

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Wednesday 04/10/13

Posted on April 10, 2013 by admin

10:00 A – 7:30 P, 10:00 P – 11:00 P Review writing working on figure 3, mixed models Revising text Cell culture diluted large flask of Kc cells plated 10 60mm plates of S2 cells for Joe. cells too dense. … Continue reading →

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Tuesday 04/09/13

Posted on April 9, 2013 by admin

10:30 A – 7:50 P, 9:30 P – 11:00 P (remotely) General mail passport for renewal for ISDB conference in Cancun helping with Will’s graduation hat Got HHMI paperwork (finally), Filed tax returns Transcription Review switched to complete numeric form … Continue reading →

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Monday 04/08/13

Posted on April 8, 2013 by admin

9:45 A – 7:30 P General Lab meeting (notes) Gel Free Electrophorysis commercial setup testing ULR Generuler ladder in low weight and high weight cut-off bags. Ran separation for 15 min at 80 V Testing on 5% gel Melted 5% … Continue reading →

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