Author Archives: admin

Protected: group meeting 03/04/13

Posted on March 4, 2013 by admin

There is no excerpt because this is a protected post.

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Sunday 03/03/13

Posted on March 3, 2013 by admin

10:00A – 1:00P, 6:00P – 7:45P, 9:00P – 11:00P Embryo Section staining Control test with AATAT worked. basic protocol No 92C pre-denature 60C 25 min minimal cooling gap. Add probe to slide, drop coverglass on, seal 94C heat-block 2.5 min … Continue reading →

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Saturday 03/02/13

Posted on March 2, 2013 by admin

10:00A – 7:00P, 8:30P-10:30P Writing stuff Revised Banff Invites letter sent to co-organizers write to van Steensel about R code Review/referee article read clean through manuscript wrote up all critiques. To do: write up manuscript strengths and contributions to field. … Continue reading →

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Updating Web Notebook: version management

Posted on March 1, 2013 by admin

Methods for version management: WP Document Revisions adds a new tab to main side widget (below Pages and Comments), called Documents. Under this tab you can upload files from your computer. These now behave has posts. Instead of editing the … Continue reading →

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Friday 03/01/13

Posted on March 1, 2013 by admin

8:10 A – 8:10P, 9:30P – 11:00P Confocal helping scan Hazen’s sections Embryo section staining BXC DFISH NO BX-C staining detectable after post-hybe hot-wash (50-60C in waterbath, 25 min). Repeat staining, try short coldwash and then check cells for signal. … Continue reading →

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Failures update

Posted on March 1, 2013 by admin

Fly Genetics Failed knockdown: 2x Mat alpha tub driving any of the TRiP valium 20 or valium 22 PcG target shRNAs. Gives viable F1 and F2s or infertile F2s. No PcG phenotypes. Kill all current crosses Kill all expanding Gal4 … Continue reading →

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Demo Gist-based version management

Posted on March 1, 2013 by admin

Protocol: Oligo Paint Synthesis permalink to most recent version. permalink to the version as it stood when this demo was published on 03/21/13. Gist embedded version of date-specific permalink using native Gist embed interpreter for markdown: [easyembed field=”Gist1″]

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demo: version managed pdf protocol

Posted on March 1, 2013 by admin

Protocol Qiagen Miniprep (QIAprep) Latest Updated version. Date specific version

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Thursday 02/28/13

Posted on February 28, 2013 by admin

10:20 A -6:45P, 8:15P – 11:55P (workout 8:30-10:20A) Probe Making (1P-6P @ Longwood) emPCR for 100kb yellow, green and black regions (at Wu lab) see new protocol 35-50 ng/uL yield (11 uL total volume) Notebook Looking in to version managing … Continue reading →

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Large Scale PCR for probe synthesis

Posted on February 28, 2013 by admin

Part 1 – PCR amplification Notes: We recommend setting up 10 ml of PCR master mix and aliquoting it into a 96-well plate, 100 µl per well. This protocol is scaled for a starting amount of 10 ml; simply scale … Continue reading →

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