Author Archives: admin

(Wed and Thur in NYC for Dale Frey interview) 9:30 am – 7:00 pm sequencing data processing bowtie finished running on all the datasets (20) Need to run cufflinks. first need to sort sam data using sam tools (should have … Continue reading →

9:00 am – 7:30 pm Library building code fixed bugs in library design — can’t call TRDesigner with parallel pool with large off-target libraries. it takes a ton of time and a ton of memory to replicate these large OTTables … Continue reading →

9:15 am – 6:00pm, 8:00pm – 8:55 pm Library building goal: building 10 kb tiling regions of BX-C probes against genome approach: adapt new OligoArray-free pipeline from transcriptome libraries to design genome libraries Problems crashed Morgan when all 256 GB … Continue reading →

12:00 pm – 6:30 pm, working on applications Submitted all apps with Nov 1st deadlines.

9:30 am – 5:00 pm Applications working on Harvard MCB application applications won’t submit requests to letter writers until application is complete. Princeton application also won’t submit requests to letter writers given that a motivated candidate would like to have … Continue reading →

9:00 am Deep seq final prep running tape-station samplesname, conc pg/uL, index, tube Ph1, 496, 1, A M1, 442, 2, A M2, 665, 4, – 10-22 #1, 652, 1, B 10-22 #2, 516, 2, B 2W, 617, 3, B 2P, … Continue reading →

9:30 am – 7:00 pm, 9:00 pm – 11:00 pm Deep seq prep continued with deep-seq prep finished second strand synthesis ran clean up on DNA-XP beads from Bauer ran adaptor ligation. premixed adaptor and enzyme mix (2 min on … Continue reading →

8:30 pm – 11:15 pm RNA deep-seq prep sample order (8 samples): 10-22 n1, 10-22 n2, 2W, 2P, 2-4W, 2-4P, 4W, 4P running sample isolation by magnetic polyT beads, following manufacturer’s protocol. 3 samples dehydrated in last elution step. I … Continue reading →