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analysis cell culture cell labeling chromatin cloning coding communication confocal data analysis embryo collection embryo labeling figures fly work genomics hb image analysis image processing images in situs Library2 literature making antibodies matlab-storm meetings modeling MP12 mRNA counting Ph planning presentation probe making project 2 project2 result results sectioning section staining shadow enhancers sna snail staining STORM STORM analysis troubleshooting writing-
GitHub Projects
Author Archives: admin
matlab-storm restructure
To do Push current individual branches to distinct user specific remotes (on user specific remote accounts). Reconfigure remotes on local repository so both the release and the development are present. This is a single repository on the local computer and … Continue reading
Wednesday, 01/01/2014
1:30p – 9:10p, remotely, 12:30a – 1:15a Goals more Ph imaging Wt New Year’s letter website updates Troubleshooting drift correction curves. start working on dual-color ChromatinCropper STORM for Ph project The buffer has all gone, because I ran the thing … Continue reading
Tuesday 12/31/13
9:00a – 5:00p Goals set up STORM imaging for PhWtPh. improved drift correction write quick script to average bead dax movies (and split them out?). Test on the G1-G1 0004 data? modify feducialDriftCorrection function to be able to handle time … Continue reading
Monday 12/30/13
9:20a -7:00p Goals Analyze 2-color STORM data test out new image-writers for hal Getting started Monet windows explorer froze and got killed. Requires restart. Finish up notes from yesterday, add gel images to notebook. STORM attempted software update to do … Continue reading
Sunday 12/29/13
9:00a – 12:15a Goals Write to Ajaz about new plans Read in _oligo.txt files and analyze probe distributions STORM G1-G2 Start analysis of F12-G01 and F12-G11 data continue work on STORMrender Manual Literature Reading Kieffer-Kwon et al Cell 2013. link … Continue reading
Posted in Summaries
Tagged coding, images, literature, probe making, STORM
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Saturday 12/28/13
9:30a – 1:45a Library 3 Design / probe building Results from first attempt at probe assembly oligoArrayCommand was getting rewritten in the loop, need a temp parameter to preserve the genename flag to be used for substitution consequently probe building … Continue reading
Friday, 12/27/13
9:30a – 9:50p Goals Test run of library 3 design. See how many probes we are up to. Test last round of 2 color stains STORM STORM imaging of F12-647, G1-750. Both dyes nice and bright. Background a bit high … Continue reading
Posted in Summaries
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Monday 12/23/13
11:00a – 5:00p chromatin project New imaging chambers working on constructing new microfluidic chambers (Jeff’s design) to refresh buffers during imaging and reversibly bind coverglass. issues getting the Plasma cleaner to work properly. Setting high? Determine oxygen flow by hand? … Continue reading
Posted in Summaries
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Sunday 12/22/13
9:45a – 11:50p Goals align 750-647 G1 double stains matlab-storm STORMrender update menu options for feducial drift correction to allow finding the bin file of feducial data using the browser (oops, this was already the default for an empty input, … Continue reading
Protected: two-color chromatin STORM controls
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