Author Archives: admin

Friday 12/13/13

Posted on December 13, 2013 by admin

10:45a – 12:15a Notebook backed up notebook updated wordpress Z-calibration cross correlation doesn’t improve curve alignment visibly — curves are fundamentally a bit different no spatial structure evident Ph Project reviewing simulation results saved as simulationName_2.eps to file Test new … Continue reading →

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Thursday 12/12/13

Posted on December 12, 2013 by admin

10:45a – 11:00p Ph Simulations Troubleshooting extra along bonds. Oops, actually just resulted from plotting the wrong attachment matrix for the given position list. Simulations ran at subsaturating Ph — more total sites than available Ph. Hence there is a … Continue reading →

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Wednesday 12/11/13

Posted on December 11, 2013 by admin

11:15a – 11:45p Ph Project Highly variable radius of gyration measurements for case with binding — suggests not equilbirated. Binding strength probably much to high. Up to .90, run again. Also increased density of K27me3 sites from 50% to 80%, … Continue reading →

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Tuesday 12/10/13

Posted on December 10, 2013 by admin

(finally recovering from flu) 11:00a – 9:00p Probe making run denaturing gel G5 looks weird – what’s with the double band?! maybe give this a test anyway. primers are different lengths, but that doesn’t explain why both primer sets have … Continue reading →

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Protected: project 2 update 12/10/13

Posted on December 10, 2013 by admin

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Friday 12/06/13

Posted on December 6, 2013 by admin

9:50a – 10:45p Goals Presentation prep for meeting with XZ (meeting rescheduled) Coding: work on new z-calibration algorithm add run silent options to STORMrender update template ini and xml parameters to contain new ROI defaults Probe Making HPLC cy7 secondary … Continue reading →

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HPLC 12/06/13

Posted on December 6, 2013 by admin

DNA labeling 60 nmol of DNA in 100 uL .1M NaHCO3 (100 mg in 12 mL) .1 – .2 mg dye in 50 uL DMSO (50 uL of 1-2 mg / mL) React at 37C for 1-2 hrs (shake occassionally). … Continue reading →

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Protected: Jeff Practice Talk

Posted on December 6, 2013 by admin

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Thursday 12/05/13

Posted on December 5, 2013 by admin

9:30a – 12:20a Goals Restart analysis for project 2 Probe making: check PCRs, continue if good, restart if not. launch STORM analysis for last few days of imaging project 2 see notes. Chromatin Project Probe Making Test PCRs Clean, reorganize, … Continue reading →

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Protected: project 2 update

Posted on December 5, 2013 by admin

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