Author Archives: admin

Sunday 11/03/13

Posted on November 3, 2013 by admin

7:30p – 10:10p STORM Analysis E08 failed to run properly through split-dax launch STORM analysis. Running smooth at 70% on Cajal. Probably close to max read/write speed for disk drive across net. Wait to launch E08 again until tomorrow. Cell … Continue reading →

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Saturday 11/02/13

Posted on November 2, 2013 by admin

9:00a – 12:00p remotely. 12:00p – 12:00a Goals Coding Add to ChromatinCropper export labeled overview fig, object centroid and bounding box in original coordinates (for computation of distance to lamina) direct measure of distance to lamina New functionalities for ChromatinCropper … Continue reading →

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Friday 11/01/13

Posted on November 1, 2013 by admin

10:00a – 9:15p Coding ChromatinCropper Current version not saving vlists! (molecule lists specific to ROI). need more display images in analysis. Data analysis: H:\2013-09-24_F6-405\splitdax Savefolder: ‘C:\Users\Alistair\Documents\Research\Projects\Chromatin\Data\2013-11-01_coloredRegions’ SplitDax System now checks filesize of the new daxfile. If expected size = actual … Continue reading →

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Thursday 10/31/13

Posted on October 31, 2013 by admin

9:30a – 10:00p STORM Stop O/N STORM of E12: 630 kb G transfer data to ProBox 4 Running out of disk space again. Order 2x 4Tb HDs Set up O/N STORM G9 (75kb B) Rowland Inst (10a – 2:30p) Seminar … Continue reading →

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Protected: Drosophila Transcription Projects

Posted on October 30, 2013 by admin

There is no excerpt because this is a protected post.

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Wednesday 10/30/13

Posted on October 30, 2013 by admin

9:00a – 10:50p STORM Finish O/N E10 imaging Hao imaging set up O/N imaging of E12 (600 kb G) with 20:30,000 bead dilution. Qualitative observations: some G regions quite large, seem pretty decompact. Others more compact? Laser power dropped to … Continue reading →

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Ph Project To Do

Posted on October 30, 2013 by admin

Goals: Counts co-localized vs. not co-localized in Mutant vs Wildtype Psc knockdown and imaging More numbers for Ph in Ph-Wt and Ph in Ph-M Modeling: a. get correct random walk behavior from polymer model b. reproduce data trend with ka … Continue reading →

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Tuesday 10/29/13

Posted on October 29, 2013 by admin

9:30a – 10:00p Other Goals Spoke broke. Pick up repair between 4p-6p. Presentation Prep working on slides for Rowland Center Talk. STORM Finish O/N imaging of E08 (75kb R) Set up imaging of E10 (100kb Y) Mentoring HC genes for … Continue reading →

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Monday 10/28/13

Posted on October 28, 2013 by admin

10:00a – 8:30p Goals reboot Tuck/Cajal disconnect all external drives and power supplies. Clean-up all cables for Tuck/Cajal. Check STORM4 run. Work on Slides for Rowland Center Talk on Thursday. Run genes for Hao STORM4 Lost focus somewhere. Looks like … Continue reading →

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Sunday 10/27/13

Posted on October 27, 2013 by admin

5:30p – 10:15p STORM Record z-calibration scans for STORM4 Imaging E1 (200 kb green) cells on STORM4 Chromatin modeling Found small bug in Bogdan’s code which prevents condensation This seems to now to work as expected. Data copying data from … Continue reading →

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