Category Archives: General STORM

Z-calibration

Posted on February 3, 2013 by admin

Same set of molecules at a collection of different heights Challenges Stage tilted: all molecules in a given frame DO NOT have the same wx wy distortion. Stage started position uncertain — might not be mid-range. Stage z-drift during movie … Continue reading →

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Coding Goals 02/02/13

Posted on February 3, 2013 by admin

Short Term Goals DaoSTORM Clean up calls for DaoSTORM in STORMfinder to use simplier DaoSTORM installation. (Complete 02/02/13) Test run DaoSTORM Merge all active projects in Matlab_STORM into Beta Move latest version of Insight3 and DaoSTORM in matlab-storm. (completed 02/02/13) … Continue reading →

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Saturday 02/02/13

Posted on February 2, 2013 by admin

11:30 A – 1P, 1:45P – 12:45 A STORM Overnight run on STORM3 crashed at around 34 of 85 movies — ‘memory error’. send crash report to Hazen. copying data form Alistair9 to Alistair8. Imaging BX-C embryos with H3K27me3. One … Continue reading →

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Coding goals ~01/26/13

Posted on January 26, 2013 by admin

Install Readme for DaoSTORM (which files do we need to copy) compiled binaries for 64-bit windows installation and filepath setup for MinGW-64bit See if Homotopy Install guide works.  If so similar approach should also get DaoSTORM running. New molecule list … Continue reading →

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STORM2 BX-C 01/23/13

Posted on January 23, 2013 by admin

Setup Back-optics aligned Focus lock working Laser power: 647 laser at 200 mW source: 130 mW at power meter 405 laser: max power = 1.89 mW.  10% = .09 mW z-stage drift without focus lock is ~800 nm per min … Continue reading →

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Code meeting

Posted on January 19, 2013 by admin

Organization Functions ‘Clean code’ * has help file — lists all inputs and outputs with descriptors functions have small number of mandatory inputs (typically 1-4) additional inputs are passed as ‘Flag’,value pairs.  (see examples) long code is written in demarcated code blocks … Continue reading →

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STORM2 session 01/11/13

Posted on January 12, 2013 by admin

Laser powers 750 at 1700 mA is 209 mW at meter 647 at 200 mW source is 120 at meter.  300 mW source is 190 at meter 488 at 75% power is 200 mW at meter 405 at max is … Continue reading →

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STORM2 11/24/12

Posted on November 24, 2012 by admin

Laser powers 405: 1.5 mW 488: 115 mW at 45% power 561: 135 mW at 200 mW GUI/laserhead 642: 135 mW at 200 mW GUI/laserhead 750: 250mW at 1800 mA. –> too high? 750: 120 mW at 1400 mA –> … Continue reading →

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Protected: Notes – Lab meeting 11/19/12 Graham

Posted on November 19, 2012 by admin

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STORM2 rebuild SOP

Posted on November 19, 2012 by admin

  Follow text file SOP for 750 laser. Aligning back optics place microscope in epi mode (12.000) Course tuning remove 20x, 100x objectives. screw in target objective in 100x objective position. remove final lens before scope body, add iris in … Continue reading →

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