Category Archives: Journal Club

Journal club presented by Guisheng In Vivo Expression of a Light-Activatable Potassium Channel Using Unnatural Amino Acids using un-nautral ammino acids to make irreversibly inducible potassium ion channels. not clear how this is really an improvement or has advantages over … Continue reading →

Doory Ultrafast endocytosis at mouse hippocampal synapses Nature 504, 2013 Background lab previously published correlative EM Combine optogentics and high-pressure freezing ‘flash and freeze’, to study synapse. 40 nm EM sections methods of exocytosis full fusion (vesicle fuses and releases) … Continue reading →

Sleep clears metabolites from the adult brain Colenso lab journal club Consensus that sleep is restorative, little mechanistic understanding of why. Lymphatic systems does not extend into CNS / brain. (in other part of the body used to clear fluid) … Continue reading →

(Sebastian presenting) Paper Lang … Chin Nature chemistry 2012. currently method of choice in the field. background site specific labeling of single cystine. Hard to remove extra Cys Fluorescent protein fusions (large, may have major functional impacts). self-labeling proteins: SNAPtag, … Continue reading →

Steven presenting CRISPR cell paper One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering Background CRIPR repeat sequences separated by spacers. Spacers are different, match different bacterial phage sequences. components: Cas1, Cas2, Csn2 (involved in encorporating … Continue reading →

Ke presents: A tissue like printed material by Villar, Graham and Bayley background multiple lipid droplets form lipid bilayers at their interface. can arrange droplets in arrays manually, (droplets on 500 um diameter scale). develop 3D printing strategy for assembling … Continue reading →

CLARITY hydrogel electrophoretic tissue transmutation. Nature Paper cross link gel. Add ionic detergent (SDS micelle), extract all membranes in electrophoresis tissue becomes much clearer, can image deep proteins still there. do they demonstrate nucleic acids? – doesn’t look like it. … Continue reading →

Probing Meiotic recombination and aneuploidy single sperm cells (Xie lab) haplotyping homologous recombination, major source of genetic diversity determine haplogenotype of each cell. Sequence diploid, not sure which SNPs are on which homologous copy (i.e. which are linked) HAP map … Continue reading →

Aberrant light directly impairs mood and learning through melanopsin-expressing neurons LeGates et al 2012 (presented by colenso) Circadian rhythm Natural pacemaker controls circadian rhythm. 50% of blind individuals experience ‘free running’ – no entrainment to light. about a 25 hr … Continue reading →