Category Archives: Fly Work

Protected: SCM summary

Posted on November 16, 2012 by admin

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Thursday 11/15/12

Posted on November 15, 2012 by admin

9:15 A — 6:45 P, 8:30P – 12:00A collect virgins for sim Espl[D] recombineering new cross, sna males x Sp/CyoZ; TM2/TM6 virgins clear MTDs and TM2/TM6s for virgin collection (peak emergence for latest bottles?) pulverize and refreeze Espl[D] and sim[D] … Continue reading →

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Friday 11/09/12

Posted on November 9, 2012 by admin

9:30 A – 8:30P Making double mutants Collect virgin TM2/TM6 flies Clear Pr/TM3,hbZ and Sp/CyOZ for virgin collection to remake double balanced 2,3 flies. TM6b = AntpHue1 . TM2 = Ubx130esemc2 . E(spl) and sim[D](7646) both have TM6b, Tb.  Should be lethal with TM6. … Continue reading →

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Friday 11/02/12

Posted on November 2, 2012 by admin

9:30 A – 7:48 PM, 10:50 – 11:45PM Fly work Flip collection plates on MTD x SCM, MTD, and sim[D], 9:30A Flip collection plates, sim[D] 1:15  P Collect virgin Act5-Gal4, MTDs, and sim/Espl.  Sort sim/Espl against humoral bristles. New crosses … Continue reading →

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Friday 10/12/12

Posted on October 12, 2012 by admin

9:45 A – 7:00 P, 8:00P-9:15P Fly work collect virgins: E(spl)[D], sim[D], MTD new bottle cross: MTD virgins x male TRiP Scm shRNA 5 new vials cross E(spl)[D] x sim[D] collect PM virgins E(spl) and sim MTD moved back to … Continue reading →

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Monday 10/08/12

Posted on October 8, 2012 by admin

10:45 A – 6:00P, 8:00P-9:00P Fly Work Flip fly stocks clear MGD bottles for virgin collection clear sim[D] and Espl[D] for virgin collection. start expanding double-balancer stock collect virgin MGD flies Coding remove save data troubleshooting scripts from STORMrender (dramatically … Continue reading →

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crosses for E(spl) sim double mutants

Posted on September 3, 2012 by admin

Crosses: Large cross of: sim[D]/Tm6b,Tb  x Espl[D]/Tm6b,Tb take non-Tb progeny and cross to double balanced 3rd (e.g. Tm3/Tm6) Cross individual F2s to double-balancer on 3rd.  Expand these single fly lines (1 generation should be enough).  PCR across the deletions to … Continue reading →

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Tuesday 08/14/12

Posted on August 14, 2012 by admin

9:15 A – 7:00 P, 8:30 P – 11:20 P Finish O/N STORM run of Antp/Tub bead images z-calibration first iteration looks horrible.  May need new few guess parameters.  Attempting multiple iterations. simulating snail patterning with different promoters.  Need substantial … Continue reading →

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Wednesday 07/11/12

Posted on July 12, 2012 by admin

10:10 A – 5:ooP check antibody locations with KE in situs day 3 Confocal: tub and Ant-C genes look good.  Preliminary data suggests loci mostly paired in older embryos but not condensed — substantial separation distance. Ubx and AbdB have … Continue reading →

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Wednesday 06/20/12

Posted on June 20, 2012 by admin

10:50 A – 6:30P ordered 0.04 um Fluospheres (540 + 715).  large fluospheres give unusable 3D maps No 715 Fluospheres, ordered 20-nm 705 quantum dots Fixed error in Z-cal convergence.  Modified convergence measure to use both x and y data. … Continue reading →

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