Category Archives: Chromatin

Protected: First look at embryo clustering data

Posted on February 22, 2013 by admin

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Protected: cluster analysis first try

Posted on February 18, 2013 by admin

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Oligo Paints Goals 01/30/12

Posted on January 30, 2013 by admin

In situs single color BX-C-cy5 in S2R+ cells (done) BX-C-cy5 in S3 cells (image: 01/30/13) Fab7-cy5 + 405 activator in S2R+ (image: 01/31/13?) Ubx-cy5 in S2R+ (?) Abd-B in S2R+ cells (not yet stained) Abd-B in S3 cells (not yet … Continue reading →

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BXC + H3K27me3 first try

Posted on January 27, 2013 by admin

  K27me3 post-in situ labeling turned out pretty sparse.  Still have a few thousand localizations for which cluster nicely in several cases at the BX-C locus.   Overall this 488 staining is very sparse, there are maybe an order of … Continue reading →

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Protected: BX-C clusters first look

Posted on January 26, 2013 by admin

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Saturday 01/12/13

Posted on January 12, 2013 by admin

10:30 A – 8:00P Probe making precipitate probe, resuspend in fresh DNAse free ddH2O snail data analysis run nuc mapping cyto2nucmap for MP08Hz_snaD_2 data modify lsm_viewer to not append embryo numbers when running export all on embryos data without position … Continue reading →

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Thursday 12/13/12

Posted on December 13, 2012 by admin

10:15 A -7:00P, 8:00P – 9:45P, STORM imaging TIRF controller crashed.  Will not revive. After hours of various attempts and help from several lab members, Graham finds in manual that power cycling the controller fixes the problem.  Unplug the black … Continue reading →

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Testing small oligopaint probes

Posted on November 10, 2012 by admin

First small test probe Reagents The primer  has a 5′ TYE665 dye. Both primers are at 200 µM (200 pmoles/µl).  template was at 10 ng/µl. A look at the genomic region Zoom in on region   Question for Ting /Brian … Continue reading →

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Protected: Notes from meeting with Welcome Bender

Posted on November 9, 2012 by admin

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Protected: Francis Lab Meeting feedback

Posted on November 7, 2012 by admin

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