Monthly Archives: February 2011

Protected: MP08 rescue (no shadow)

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Thursday 02/17/11

9:10 A — 7:05P, 7:50P – 11:00P Finishing draft presentation for practice talk. wash, stain nuclei, and mount slides (in situs). collect virgins Flip collection plates 5:20P (fog and fog; Hkb-fog). Reorganizing presentation flip fly stocks Confocal, MP08 rescues Confocal … Continue reading

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on statistical testing

Reviewer asserts “the appropriate test for these particular data is a two-way ANOVA, (but be careful when analyzing proportions.  The data should be arc-sine transformed to minimize deviations from the assumption of normality). ANOVA has long been used to classify … Continue reading

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Wednesday 02/16/11

11:00 A — 4:oo P, 9:20P – 10:00P in situs day 3. Post-doc interview (from Long Lab) collect virgin DB BAC-4s and DB hb[D]s Recover A. Mori data for Ciona team Biophysics Dinner collect virgin DB BAC-4s and DB hb[D]s … Continue reading

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Tuesday 02/15/11

10:30A — 7:00 P, 10:30P-12:30A in situs day 2.  Using heat block for hot washes GP a-twi antibody for Bilder lab (re-suspend in 50% glycerol). Flip collection cages 12:30P.  All fog lines hardly laying at all. clean up im_quant_sna_v3.fig for Jen … Continue reading

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Monday 02/14/11

10:00A — 12:30 A 11A, Stop confocal run: (only finished 10 embryos) Collect virgin DB04 and DB Hb[D]s Biophysics training grant strategy meeting. in situs day 1B. book hotel at Sloan, (Bentley Hotel 500 E. 62nd St., 888-664-6835) conf-rv: 11828 … Continue reading

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3D-dot segmentation.

Working on 3d dot visualization, check duplicate dot counting code and explain method.  These images are endogenous snail from MP09 imaging.  Gaussian sphere pretty good, most dots successfully caught in 3 layers using 340nm steps.   Nice yellow center to … Continue reading

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Sunday 02/13/11

12:45 P – 7:00 P, 9:00P — 12:10 A Finish confocal run (MP10, slide from 2/2/11 in situs) Collect virgins from DB04 cross. Analyzing new MP10 data New fog- collection cage.  fog flies ready to collect virgins and cross to … Continue reading

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Saturday 02/12/11

10:30 A – 8:00P, 9:00P – 11:35 P Write to Ptashne about additive vs. cooperative (vs multiplicative). Finish first draft of reviewer comments and send to Peter and Steve. Writing new imviewer_lsm GUI.  Now functional. Heating up lasers in first … Continue reading

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Protected: Comparing tiling data to counting data

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