Monthly Archives: March 2011

Friday, 03/25/11

Posted on March 25, 2011 by admin

10:30 A – 9:20 P Making figures for Fly Meeting presentation. Figure ‘pol pausing as a checkpoint’ for ML Found bug in CheckDotUpDown.m while making methods figures. (Causes missing of stacked dots) Suspected this might exist, need to re-run dot-counting. … Continue reading →

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Protected: new hb analysis

Posted on March 25, 2011 by admin

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Thursday, 03/24/11

Posted on March 24, 2011 by admin

10:10 A – 6:20 P, 8:00P – 12:35 A Playing with Data: troubleshooting dotfinding and doing parameter sensitivity with MP10_e data. Trying to make slides for Fly Meeting. Figures for Mounia + vector graphics fix fig 4 for Ecology paper Good article … Continue reading →

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Wednesday, 03/23/11

Posted on March 23, 2011 by admin

10:30A –8:00 P, 9:00 – 11:00P Analysis of mRNA decay (see post).

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Protected: Transcription kinetics

Posted on March 23, 2011 by admin

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Tuesday, 03/22/11

Posted on March 22, 2011 by admin

10:00 A – 9:10 P Confocal: MP10_22C from 02/06/10 slides: 3 mitotic embryos for attempt to measure mRNA lifetime. More analysis of effect of TF concentration and enhancer number of activation probability using Dorsal > snail and Bcd > hb. … Continue reading →

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Monday 03/21/11

Posted on March 21, 2011 by admin

10:30 A — 8:00P, 10:30P — 1:20 A MP09_e data run finished.  Set up MP01 data run (map nuclei and run unsupervised_dotfinding). Moving _nucdata.mat files into appropriate rawdata/subfolder instead of Enhancer_modeling data. New code: Plot_DotComps2_ConcDept_hb, uses entirely rewritten divide regs … Continue reading →

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Sunday 03/20/11

Posted on March 20, 2011 by admin

10:35 A — 6:30P Communication with Sanjay Tyagi and Arjun about steady state solution to master equation for simple mRNA synthesis decay model.  Dan Tranchina has full derivation (not yet published). MGa2x_b ON analysis seems to have completed smoothly.  Starting … Continue reading →

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MP05 MP10 comparison

Posted on March 19, 2011 by admin

Summing up results from dot counting: From fxn_regionvar, tight boundaries (to remove edge effects) (mostly thresh = .6) MP10 emb, snail mean, var,  reporter mean, var 06, 247, 23,  155, 17 08 206, 25, 129, 17 07 222, 25,131, 18 … Continue reading →

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Saturday 03/19/11

Posted on March 19, 2011 by admin

11:15A — 7:30 P, 8:30 P – 11:30 P Finish O/N confocal (only 11 embryos complete) Troubleshooting imviewer_lsm project.  Need to rewrite to write blank files for skipped rows. Working on MP05 MP10 total dot comparison. Working on enhancer switching … Continue reading →

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