Monthly Archives: December 2011

Sunday 12-04-11

11:00A Continuing running O/N snail imaging of 16 wt embryos (finishes about 5P) Analyzing snail mRNA counting data, manually checking dot counts. offset centroids lead to errors in z intensity (would be better to find the peak value of all … Continue reading

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Saturday 12-03-11

9:30 A –12:00A Finish O/N confocal run, all 9 embryos completed.  Most are early cc14 Analyzing O/N dot counting on old data with revised multilayer and peak-intensity checker.   Basic counts all in the same range, some gradients a bit … Continue reading

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Friday 12-02-11

9:30 A – 1:00 A Finish O/N run of wt snail embryos (6p-10p with 1hr warmup, runs 10.5 embryos) analyzing new snail dynamics data (see protected posts) set up new O/N run of wt snail embryos (9 embryos, 8p-10a)

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Protected: more early snail dynamics

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Protected: snail dynamics

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Thursday 12-01-11

10:30 A — 11:00P Testing WGA labeling after Xylene treatment with WGA in PBS and WGA in block. Steve Chu lecture Analyzing new MP08, MP05 data. Running new MP06 data apparently WGA in block does not bind substantially to membranes. … Continue reading

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Protected: New MP08 snaD data

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Protected: H3K4 imaging

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