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Monthly Archives: September 2012
Thursday 09/06/12
9:50 A – 10:00 P InsightM overloads Tuck from O/N data analysis. Canceled all further submissions to que to allow memory / CPU to free up for other users during the day. InsightM also crashing itself due to memory overload … Continue reading
Wednesday 09/05/12
9:30 A – 9:00 P sectioning 6/25 sample 10: E(Pc)-dig, en-bio, H3-750, K27-647. writing figure text for snail promoter/enhancer manipulations First coverslip of S10 most sections adhered to coverslip before chloroform treatment. may not be fully flattened. 4 coverslips cut, … Continue reading
Tuesday 09/04/12
9:30A -4:00P Take bead images for color calibration. (looks like system may have relaxed and is now clipping on the right instead of on the left? Check when next I have scope time… Temporal analysis of snail dynamics with different … Continue reading
Protected: snail figure updates
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crosses for E(spl) sim double mutants
Crosses: Large cross of: sim[D]/Tm6b,Tb x Espl[D]/Tm6b,Tb take non-Tb progeny and cross to double balanced 3rd (e.g. Tm3/Tm6) Cross individual F2s to double-balancer on 3rd. Expand these single fly lines (1 generation should be enough). PCR across the deletions to … Continue reading
Monday 09/03/12
10:00A – 6:30P, 7:50P – 11:30P Order flies from Bloomington, sim deletion: 7646, Espl rv1: 199 # start expanding sna[18] and double balancer on 3rd. request Esc mutant flies from Vivek wrote to Markstein, try to get in touch with TRiP … Continue reading
Protected: enhancer mediated repression
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Posted in Shadow Enhancers, snail patterning
Tagged images, result, sna
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Sunday 09/02/12
11:30 A – 3:00P, 5:30P-8:00P writing to mounia about sna ths getting data for mounia on sna ThsPr: cc14l, cc13 writing to Mike / Jacques / Mounia about papers m116a & b has a bit high false detection rate (higher … Continue reading
Posted in Uncategorized
Tagged coding, communication, image analysis
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Saturday 09/01/12
4:45 P – 7:00P Break down STORM4 O/N run on S1 AbdA AbdB Ubx Break down confocal O/N run of sog Pr. Analyzing snail Pr Ths Pr data. Fixed bug in snail_comp_promoters code: index i was being used both in … Continue reading