Monthly Archives: April 2013

Wednesday 04/10/13

Posted on April 10, 2013 by admin

10:00 A – 7:30 P, 10:00 P – 11:00 P Review writing working on figure 3, mixed models Revising text Cell culture diluted large flask of Kc cells plated 10 60mm plates of S2 cells for Joe. cells too dense. … Continue reading →

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Tuesday 04/09/13

Posted on April 9, 2013 by admin

10:30 A – 7:50 P, 9:30 P – 11:00 P (remotely) General mail passport for renewal for ISDB conference in Cancun helping with Will’s graduation hat Got HHMI paperwork (finally), Filed tax returns Transcription Review switched to complete numeric form … Continue reading →

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Monday 04/08/13

Posted on April 8, 2013 by admin

9:45 A – 7:30 P General Lab meeting (notes) Gel Free Electrophorysis commercial setup testing ULR Generuler ladder in low weight and high weight cut-off bags. Ran separation for 15 min at 80 V Testing on 5% gel Melted 5% … Continue reading →

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Protected: lab meeting 04/06/13

Posted on April 8, 2013 by admin

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Finite state Markov model notes

Posted on April 6, 2013 by admin

simple switching model. Two systems, 1 and 2, each two states A and B. fi Ai <==> Bi bi Bi –> Bi + mRNA A1 <==> A2 [* f1 s12 0 b1 * 0 0 s2 0 * f2 0 … Continue reading →

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Friday 04/05/13

Posted on April 5, 2013 by admin

9:00 A – 8:00 P, 9:00 P – 10:45 P General (8:00 A allergy test) STORM analysis Getting images of relative background levels in 3-Cy5 vs 405-cy5 Drift correction makes sparse images look horrible (even the rather long integration of … Continue reading →

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Protected: Activator oligoPaints: results summary 04/05/13

Posted on April 5, 2013 by admin

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Protected: issues with ph-FLAG round 1

Posted on April 5, 2013 by admin

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oligopaint-PCR

Posted on April 4, 2013 by admin

Calculating concentrations (Qubit) and molecule numbers: starting primer = 30 ng in 300 uL in 1 ng/uL, added 100 uL = 100 ng 100 uM = 100E-12 mols/uL add 100 uL, = 10 nmols starting primer. Y PCR: 73.7 ng/mL … Continue reading →

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Protected: STORM3 fab7 data

Posted on April 4, 2013 by admin

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