Daily Archives: April 1, 2013

Monday 04/01/13

10:00 A – 7:00P, 9:00P-10:00P General hp psc1200 printer not printing color correctly. lab meeting (notes) journal club (notes) Review writing Insight 1: enhancer switching between obstructed and unobstructed case is the bursting model with different tx rates from ‘off’ … Continue reading

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oligopaint probe making planning

emPCR ready libraries: AbdA AbdB Ubx en-locus 100kb Black 100kb Yellow 100kb Green 100kb Synthesis schemes: Teritaries + Nicking PCR using extended primers = Forward primer (21bp) + Secondary binding site (32 bp) Nick off R primer Denaturing PAGE Gel … Continue reading

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journal club: single sperm sequencing

Probing Meiotic recombination and aneuploidy single sperm cells (Xie lab) haplotyping homologous recombination, major source of genetic diversity determine haplogenotype of each cell. Sequence diploid, not sure which SNPs are on which homologous copy (i.e. which are linked) HAP map … Continue reading

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Protected: lab meeting 04/01/13

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