Daily Archives: April 14, 2013

Sunday 04/14/13

Posted on April 14, 2013 by admin

5:30 P – 8:00P OligoPaint Analyzing previous gel images Conclusion: something went weird, possibly with original PCR used to seed most of the second round PCRs. Plan Repeat PCRs from scratch Run diagnostic denaturing gels at each step in addition … Continue reading →

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experimental oligopaint PCR 3

Posted on April 14, 2013 by admin

Samples (1 mL scale) y nicked 2x Ya-primer b nicked 1x Ba-primer + 1 x CPPT ubx nicked 1x UBx-adapter-primer + 1x CPPT y PPT: 5 uL YA + 20 uL PPT y PPT control: 5 uL YA + 20 … Continue reading →

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denaturing diagnostic gels

Posted on April 14, 2013 by admin

Gel 1, 5% Agarose, 200 V in sodium-Borax (300 V melts gel). Gel 2, 15% Urea, 120V 2 hrs Gel 3 15% Urea gel, 120 V 50 min in 37C room Gel 4, 10% precast Urea denaturing gel, 120 V, … Continue reading →

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