Monthly Archives: May 2013

Tuesday 05/07/13

Posted on May 7, 2013 by admin

9:25 A -5:50 P Ph FLAG Processing confocal images of Ph data updated ImageShop / ExtractLSM to fix bug in multi-position loader. Ph FLAG discussion / comments: post Literature Pirrotta Insulators target active genes to transcription factories and polycomb-repressed genes … Continue reading →

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nanodrop

Posted on May 7, 2013 by admin

measurements (ng/uL). in 150 uL unless otherwise indicated Y2.5 E1 263 Y2.5 E2 81 Y1.5 E1&2 272 K2.5 E1 519 K2.5 E2 435 K2 E1 150 K2 E2 100 K2 E3 48 (2x + reblank. prev 16?!) G E1 97 … Continue reading →

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Protected: Ph FLAG and Ph FLAG M

Posted on May 7, 2013 by admin

There is no excerpt because this is a protected post.

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Protected: project 2 updates

Posted on May 6, 2013 by admin

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Monday 06/05/13

Posted on May 6, 2013 by admin

9:30 A – 7:45 P, 8:45P – 11:55 P Oligopaints Running out gradient gels (one at a time) hotter is better with mis-priming. using 20 bp PPT primer and library specific NP primer: with adapters: Check Ph stains booked confocal … Continue reading →

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Sunday 05/05/13

Posted on May 5, 2013 by admin

STORM Analysis Fixed bug in RunDotFinder that was causing DaoSTORM analysis on to correctly overwrite/rerun analysis (DaoSTORM method check had method in quotes, causing failure). Project 2 Data analysis notes OligoPaints Running 2.5 mL of PCR using PPTC primer at … Continue reading →

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Protected: project 2

Posted on May 5, 2013 by admin

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Saturday 05/04/13

Posted on May 4, 2013 by admin

5:50 P – 8:30 P Sequential staining Attempting to align images from sequential staining. Correspondence not obvious. Substantial translation and rotation, need to fix these with feducials. Probe making Run column cleanup on LC libraries after 10 round PCR using … Continue reading →

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Friday 05/03/13

Posted on May 3, 2013 by admin

10:00 A – 8:00 P, 9:00 P – 11:00 P Joint project Help Hao relocate cells from yesterday and check sequential hybe Succesfully relocated all cells. Substantial Cy3 signal still present, but much more Atto647N. Need to analyze images. STORM … Continue reading →

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Thursday 05/02/13

Posted on May 2, 2013 by admin

10:30 A General stuff reply to Olivia about quantifying nuclear concentration, mRNA FISH Need to fedex keys and forms back to U Chicago. Wrote to Matt to get acct info # Enter all accepted Banff Invites into BIRS system, including … Continue reading →

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