Monthly Archives: January 2014

Thursday 01/23/14

Posted on January 23, 2014 by admin

9:45a – 12:15a STORM analysis analyze bead data for BXC double stains analyze bead data for PhM Psc Finish O/N run of G05 quick look at ANTC data (just image based drift correction available at present) fewer localizations despite bright … Continue reading →

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Wednesday 01/22/14

Posted on January 22, 2014 by admin

9:30a – 11:50p Chromatin project Planning write up summary of yesterday’s meeting with XZ (see post) work on improving metrics for comparison of chromatin regions (see post) writing up plans for new ChromatinCropper to implement revised analysis (see post) plans … Continue reading →

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Chromatin Project, short-term goals

Posted on January 22, 2014 by admin

Issues with current data analysis In trying to get of order 100 loci per chromatin domain I’ve take a bunch of partially imaged edge of focus domains or other less well defined images (e.g. near edge of field of view). … Continue reading →

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Protected: Library2 Data Summaries

Posted on January 22, 2014 by admin

There is no excerpt because this is a protected post.

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RT smears troubleshooting

Posted on January 22, 2014 by admin

A quick look at my history of RT success to ID what started going wrong? Observations not much smearing in early samples. Incorporation rate increases with total amount of mRNA added. Using beads to concentrate mRNA increases production. Using pure … Continue reading →

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Monday 01/20/14

Posted on January 20, 2014 by admin

9:40a – 1:00a Goals send fig files to Jeff Upgrade memory on Cajal. Image ANT-C complex analyze black K100 (internal black domain) plots of internal vs. external. Lab Meeting see notes. journal club notes Computer stuff Memory installed on Cajal. … Continue reading →

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journal club 01/20/14

Posted on January 20, 2014 by admin

Sleep clears metabolites from the adult brain Colenso lab journal club Consensus that sleep is restorative, little mechanistic understanding of why. Lymphatic systems does not extend into CNS / brain. (in other part of the body used to clear fluid) … Continue reading →

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Protected: lab meeting 01/20/14

Posted on January 20, 2014 by admin

There is no excerpt because this is a protected post.

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Sunday 01/19/14

Posted on January 19, 2014 by admin

10:00a – 11:40a, 1:30p-6:30p, Prepare submission for Drosophila image competition chromatin project adding DAM-ID data and ChIP-seq, and FARIE-Seq to chormatin colors overview working on slides for meeting with XZ

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Saturday 01/18/2014

Posted on January 18, 2014 by admin

10:30a – 7:20p, matlab-storm STORMrender online Getting Jeff set up with STORMrender debugging: STORMfinder added paths in initialization, allowing matlab-storm to access depreciated versions of code in folders that weren’t supposed to be on filepath Features to add to STORMrender … Continue reading →

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