Daily Archives: February 12, 2014

Wednesday 02/12/14

9:50a – 11:59p Ph Project Cell staining hot wash post-hybe PBS wash Block 1 hour in 2% BSA primary stain rb a-Ph for 2 hrs RT, 6 hrs 4C rinse, wash in PBS, reblock, secondary stains O/N at 4C (m … Continue reading

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STORM2 stats 02/12/14

Max Laser Powers 488 laser head (100%): 580 mW 488 before expander: 275 mW 488 microscope backport: 180 mW 561 at head (2100mW in GUI): HIGH 561 laser at beam expander: 1500 mW 561 laser at backport 900 mW 647 … Continue reading

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