Monthly Archives: April 2014

Monday 04/21/14

9:00a – 6:00p, 10:00p – 12:00a Lab meeting see notes post-doc candidate interview individual post-doc candidate meetings STORM attempted to re-image G08-P3-750 G06-P1-647 sample. All 750 spots have vanished! buffer issue? poor secondary stickiness issue? Imaged L3 E08 samples using … Continue reading

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Protected: post-doc interview

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Protected: project 2: to do

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Thursday 04/17/14

STORM prism2 lost focus just after midnight (~minutes after I checked it and went home) prism2 now upgraded to STORM1. Jeff looking into peizo replacement. (How about a piezo swap with STORM4 dual-objective?) continue imaging on STORM2 sample E07 Staining … Continue reading

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Wednesday 04/16/14

9:00a – 11:45p Goals project 2 team meeting project 2 design new experiments start analysis Project 2 see post General notes wordpress site / notebook running very slow today. STORM imaged beads imaging E07 on prism2 New hybes ~4/11 cells … Continue reading

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Protected: project 2, 04/16/14

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Tuesday 04/15/14

9:30a – 11:55p Computer stuff troubleshooting Cajal 590 GTX graphics card fried installed AMD card that came with Dell not recognizing all RAM (only 40 of 48 GB recognized) Turned off, pressed all RAM firmly into DIMM slots. Now only … Continue reading

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Protected: Project 2, new analysis functions

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Oliver Rando: I: Structural biology of the yeast genome II: Mechanical disassembly and reassembly of mammalian reproduction

MNase digestion — nucleosome phasing 26 different histone modifications mapped genome wide. in 20kb of yeast chrIII chromosome conformation capture mapping 300 nm the 30 nm fiber mode solenoid model, regular 30 nm model zigzag, 3 nucleosomes / turn, less … Continue reading

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Monday 04/14/14

9:45a – 9:00p Computer issues Attempted to swap memory into Cajal. Cajal won’t boot up with any of the memory restored Tuck to 6x 8GB DIMMS. Cajal has 12x 4GB DIMMS wrote to IT to help get system back up. … Continue reading

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