Monthly Archives: May 2014

Friday 05/16/14

Posted on May 16, 2014 by admin

Chromatin Project Analyzing E06+ E07 data: chr2R:19726615-19888410__YELLOW_D12 a + b 4Tb hard-drive in Kangaroo mount died. All data from F03-F02 and F04-F02 lost. Drive recovery services probably not worth it. Damn it. chkdsk ‘recovered’ the files by deleting all the … Continue reading →

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Thursday 05/15/14

Posted on May 15, 2014 by admin

10:00am – 5:45pm Hal froze last night on movie 4! :(. Restarted hal, restarted imaging. Really should remember to check this before I leave — this must have happened before I went home last night near ~1 am, since the … Continue reading →

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Wednesday 05/14/14

Posted on May 14, 2014 by admin

8:45a – 12:45a 9:00a-11:15a, lab volleyball practice Probe synthesis B02-B09 3ul P1-A405, 10 uL RNA, plus 5 uL RT buffer, 2 uL dNTP, 1.5 uL ddH2O, 1.5 uL inhibitor, .2 ul RT Master = 27 uL P1-A405 + 45 uL … Continue reading →

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Tuesday 05/13/14

Posted on May 13, 2014 by admin

10:00a – 11:30p Project 2 see notes Chromatin Project New Probes making L3: B02 – B09 L2: F03, F04, F05, F06, F07. L3: F09, F10, F11 (all 2 color), running QPCR (try 2) over-ran a little bit, amplification happens quite … Continue reading →

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Protected: project 2 update: 05/13/14

Posted on May 13, 2014 by admin

There is no excerpt because this is a protected post.

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Monday 05/12/15

Posted on May 12, 2014 by admin

9:30a – 5:00p, 10:30p – 12:00a Goals Finish stains 2-clr STORM imaging En TAD regions Group meeting prep. Communication email Graham email Steph dates Lab Meeting (laptop battery died, no e-notes). 750 with adaptive optics? Probe making RT-PCRs didn’t work. … Continue reading →

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Sunday 05/11/14

Posted on May 11, 2014 by admin

12:00p – 5:00p Finish probe making: E05-E12, F01-F08 p3 Run gel Run clean up. New stains F02-p3, F04-p1 F02-p3, F03-p1 F03-p3, F04-p1 New Probes to make L3: B02 – B09 L2: F03, F04, F05, F06, F07. L3: F09, F10, F11 … Continue reading →

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Monday 05/19/14

Posted on May 10, 2014 by admin

9:00a – 6:00p My lab meeting feedback Complement area analysis as Radius of gyration of molecule positions. volume analysis for some loci. log transform spot densities to avoid saturation. Area comparison plots normalized to largest spot fraction of single vs. … Continue reading →

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Friday 05/09/14

Posted on May 9, 2014 by admin

Goals Project 2 relaunch batch analysis troubleshoot output send new writeup to Xiaowei STORM keep rolling. Samples to Image L2G06-p1-A647 L2G08-p1-A647 Samples to stain F01 – F06 P3 Probes to finish F01-F08 and E05 – E12 F04(EPc) + F02(En) vs … Continue reading →

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Tuesday 05/06/14

Posted on May 6, 2014 by admin

9:00a – 12:00p Conference Prep Review speaker list, read up on papers. Travel to Janelia. Project 2 see notes

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