Monthly Archives: September 2015

Protected: CONTI center notes

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Sunday 09/13/15

3:45 pm – 7:45 pm STORM C03 imaging finished ~4:00 pm (17 hours) replaced with fresh buffer, setting up new tile scan. Fixed Kc cells plated on 18mm round coverslips last night. started new imaging round started DaoSTORM running on … Continue reading

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Saturday 09/09/12

12:15 pm – 5:30 pm, 8:40 pm – 11:20 pm Ph manuscript revisions went through and main text revisions, updated and replied went through supp text revisions, updated and replied updated Supp Figs (updated SF1, check with NF about SF11 … Continue reading

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Friday 09/11/15

Manuscript paper revisions finalizing response to reviewers with BB comments discuss updated comments with XZ Figure changes to do Figure 1 Add Pc knockdown results to panels c and d (remove ED Fig d) Figure 2, no change Figure 3 … Continue reading

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Thursday 09/10/15

9:30 am – 7:00 pm Finalizing draft of response to reviewers polishing figures a bit (removing boxes, fixing axes etc) finish writing missing captions 9:30 am – 10:30 am communication catching up on communication emails (10:30 – 11:15) coffee with … Continue reading

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Wednesday 09/09/15

9:15 am – 5:10 pm, 8:00 pm – 11:00 pm Response to review letter comparisons with conventional imaging working on deconvolution went back to fully automated quantification of conventional images using half max to address thresholding — works pretty well. … Continue reading

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Tuesday 09/08/15

9:00 am – 8:00 pm Revising manuscript New versions of all the main figures and all the extended data figures now started. Figure items: Still need to do deconvolution of conventional images add anti-aliased full size conventional images analysis of … Continue reading

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Monday 09/07/15

10:15 am – 8:40 pm, 9:40 pm – 12:00 am tasks discuss quantification approaches for live data working on figures practice talk for CONTI retreat/review working on figures (see git and figure notes)

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Protected: notes 09/07/15

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Grant review

CONTI grant evaluation meeting organization first: Tako Hensch, microRNA — AGO2 is imprinted Lichtman then Dulac then us? Bogdan Talk: Chromatin structure ‘identical’ probably too strong for imprinted genes one allele is 1.5-2x larger than the other in imprinted tissue … Continue reading

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