Tag Archives: STORM

STORM3

Posted on March 19, 2012 by admin

STORM 3 setup notes 561 line: Com 8, up to 100 mW at detector when running at 300 mW at laser.  Controlled by AOTF 656 red line.  Power switch on tabel, modulated by AOTF.  up 100 mW at detector: mirrors … Continue reading →

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Sunday 03/18/12

Posted on March 18, 2012 by admin

11:00 A – 8:45 P No colonies on plates Finish O/N STORM run.  Run failed at movie 121, unknown error message. attempt bead feducial drift correction: polysterene beads good autofluoresnce in all but 750, but bleach fast. 405 beads not … Continue reading →

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Saturday 03/17/12

Posted on March 17, 2012 by admin

11:00 A – 10:00P Experimenting with heat fixing embryos working on ACS application filter extract new tracer beads.  Initial test suggests still contains raw dyes.  Tried spin. No obvious colonies on plates.  Back at 37C O/N. STORM imaging of Pho … Continue reading →

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Thursday 03/15/12

Posted on March 15, 2012 by admin

9:45 A – 9:30P minor z drift issues still observed in STORM run. Time to take vertical beads for z-fit.  at 1:1000 density is a bit low. try 1:500 using ND 2-3 for z-fit bead images. Might be saturated?  Need … Continue reading →

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Sunday 03/04/12

Posted on March 4, 2012 by admin

10:40 A – 10:30 P Working on color registration for S2 data Laying out motivation slides for Fly Meeting presentation Working on fly meeting slides Fixed STORM2: 405 laser not working required Hal reboot. Reinstalling square aperture required bead slide … Continue reading →

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Friday 03/02/12

Posted on March 2, 2012 by admin

10:05 A -1:00 A Flip new PcG shRNA lines. Enter into stocks. Screen MP12 rescue crosses.  set up 6 new crosses, 3 with bright eyes might be homozygous MP12. 1 vial with straight wings tossed and 1 vial with Sp … Continue reading →

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Recent STORM image gallery

Posted on March 2, 2012 by admin
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Compare 2D and 3D spot finding results

Posted on March 2, 2012 by admin

2D in green, 3D in red (H3-647).   Z images are collapsed down into single plane.  It looks like brightness differences are substantial, better contrast total photons from 2D imaging (might have expected reverse, 3D apparently doesn’t compensate for photon … Continue reading →

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Wednesday 02/29/12

Posted on February 29, 2012 by admin

9:45 A — 11:00P Washing out O/N 4C antibodies from new protein stains Flip Fly stocks Flip vials and enter new GFP lines into fly stocks. Start expanding Gal4 bcd-3′ UTR (to try crossing into PcG RNAi lines.  will need … Continue reading →

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STORM 2-28-11

Posted on February 29, 2012 by admin

Laser powers: 750: 104 mW 647 : 150 mW 561: 150 mW 488: 150 mW 405: 1.5 mW Sample 2H.  750 switching nicely. 647 very bright conventional images.  Under high laser power image still inverts — low nuc singal high … Continue reading →

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