Tag Archives: writing

Monday 10/22/12

Posted on October 22, 2012 by admin

9:20 A – 8:30, 9:30 – 11:10PM Flip collection cage take embryos out of 4C, final hour incubating at RT before washes. snail in situs Draq5 stained and mounted STORM / section in situs / DFISH all in secondaries at … Continue reading →

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Sunday 10/21/12

Posted on October 21, 2012 by admin

11:00 A-3:00P, 7:00P – 9:40 PM upgrade linux laptop to 12.10 Review writing reading /re-reading recent literature White/Barolo/Cohen paper He / Sinha model see post Experiments Rinse out probes from DNA FISH (probes saved – green box) Rinse out probes … Continue reading →

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Tuesday 10/16/12

Posted on October 16, 2012 by admin

9:00 A – 6:50P, 8:20P -11:15P discuss CMAKE minGW sys with JM 9:30 A flip collection plate Working on BioPhys J review. 11:30 A – check samples from oven substantial issues with resin quality: 1 sample R solid at 70C. … Continue reading →

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Thursday 10/11/12

Posted on October 11, 2012 by admin

9:30 AM – 7:45 P, 9:00P – 11:15 PM Fly Work Collect virgin MTDs, E(spl) and sim[D] Collect male E(spl), sim[D] (none) and Scm.  Su(Z)12 stock struggling, would be good choice for knockdown. Set up MTD x Psc (and MTD … Continue reading →

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Wednesday 10/03/12

Posted on October 3, 2012 by admin

Fly to San Jose for Damon Runyon retreat working on BJ review on transcription regulation in the early embryo

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Sunday 09/30/12

Posted on September 30, 2012 by admin

5:15P-6:45P, 8:00P-10:00P returned from lab retreat (Friday 4:30P – Sunday 2:00P). working on CR revised supplement Revised Supp Mat (with new section), Revised Supp figures, and 2 draft ‘graphical abstracts’ sent to ML for review.

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Thursday 09/27/12

Posted on September 27, 2012 by admin

9:35 A  – 10:10 P Revising text Revising figures: convert all fonts, etc Finished first complete run-through of response to reviewers,  Additional to do.  (X indicates complete) X reformat all references (opening on laptop lost references?) X check that legends … Continue reading →

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Transcription traffic jams

Posted on September 26, 2012 by admin

Selth et al Elongation Review: polII collisions yield backtracking, pol II collisions yield reactivation / push through of paused leaders.  Longer genes have more traffic jams (Swinburne 2008 G&D, see below). Saeki and Svejstrup 2009 Mol Cell Collisions can push … Continue reading →

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Wednesday 09/05/12

Posted on September 5, 2012 by admin

9:30 A – 9:00 P sectioning 6/25 sample 10: E(Pc)-dig, en-bio, H3-750, K27-647. writing figure text for snail promoter/enhancer manipulations First coverslip of S10 most sections adhered to coverslip before chloroform treatment.  may not be fully flattened. 4 coverslips cut, … Continue reading →

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Tuesday 09/04/12

Posted on September 4, 2012 by admin

9:30A -4:00P Take bead images for color calibration.  (looks like system may have relaxed and is now clipping on the right instead of on the left?  Check when next I have scope time… Temporal analysis of snail dynamics with different … Continue reading →

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