Tag Archives: fly work

Saturday 10/13/12

Posted on October 13, 2012 by admin

11:00A – 6:ooP, 9:ooP-11:00P binomial statistics and confidence intervals discussion with JM Collect virgin E(spl) and sim[D] flies, 4 more vials of crosses. Finished virgin collections and cross-setups for present.  All flies now on RT shelf.  Organizing Matlab STORM library … Continue reading →

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Friday 10/12/12

Posted on October 12, 2012 by admin

9:45 A – 7:00 P, 8:00P-9:15P Fly work collect virgins: E(spl)[D], sim[D], MTD new bottle cross: MTD virgins x male TRiP Scm shRNA 5 new vials cross E(spl)[D] x sim[D] collect PM virgins E(spl) and sim MTD moved back to … Continue reading →

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Thursday 10/11/12

Posted on October 11, 2012 by admin

9:30 AM – 7:45 P, 9:00P – 11:15 PM Fly Work Collect virgin MTDs, E(spl) and sim[D] Collect male E(spl), sim[D] (none) and Scm.  Su(Z)12 stock struggling, would be good choice for knockdown. Set up MTD x Psc (and MTD … Continue reading →

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Tuesday 10/09/12

Posted on October 9, 2012 by admin

10:00 A – 7:20P, 8:30P – 11:30P Fly Work collect virgin MTDs.  (progeny of MTD x TRIP -> collection cage.   These embryos should lay infertile offspring / transformed embryos).  Let’s do Psc; MTD first, see if structure of K27me3 … Continue reading →

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Monday 10/08/12

Posted on October 8, 2012 by admin

10:45 A – 6:00P, 8:00P-9:00P Fly Work Flip fly stocks clear MGD bottles for virgin collection clear sim[D] and Espl[D] for virgin collection. start expanding double-balancer stock collect virgin MGD flies Coding remove save data troubleshooting scripts from STORMrender (dramatically … Continue reading →

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Tuesday 10/02/12

Posted on October 2, 2012 by admin

9:15A – 8:00P, 9:00P-11:00P Drop-off poster USB at MCB graphics for printing (using FAS secure file delivery) Discussing fixation and reduction with Jeff, sent Drosophila and cell fixation protocols. Pick up printed poster Pick up Drosophila from Bloomington from mailroom … Continue reading →

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Wednesday 09/19/12

Posted on September 19, 2012 by admin

9:30 A – 2:45P Working on GUI somehow the zoom button isn’t getting passed the correct minx and miny, even though these sit in the handles structure and it is getting passed the correct handles.zm !!!! WTF. Rest of navigation … Continue reading →

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crosses for E(spl) sim double mutants

Posted on September 3, 2012 by admin

Crosses: Large cross of: sim[D]/Tm6b,Tb  x Espl[D]/Tm6b,Tb take non-Tb progeny and cross to double balanced 3rd (e.g. Tm3/Tm6) Cross individual F2s to double-balancer on 3rd.  Expand these single fly lines (1 generation should be enough).  PCR across the deletions to … Continue reading →

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Wednesday 08/15/12

Posted on August 15, 2012 by admin

9:45 A – 6:50P, 8:00P – 11:20PM Finish O/N confocal run on slide m105, save data running simulation on full size embryo of snail promoter mutants.  Strong preference for patches.  Earlier activation of repressors supports conditions that create broader snail … Continue reading →

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Tuesday 08/14/12

Posted on August 14, 2012 by admin

9:15 A – 7:00 P, 8:30 P – 11:20 P Finish O/N STORM run of Antp/Tub bead images z-calibration first iteration looks horrible.  May need new few guess parameters.  Attempting multiple iterations. simulating snail patterning with different promoters.  Need substantial … Continue reading →

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