Tag Archives: chromatin

Thursday 07/18/13

Posted on July 18, 2013 by admin

9:30 A – 7:45 P, 8:30 P – 12:00 A expanding primer sets looking into quaternary cyclic codes to generate primers with appropriate distance There is literature on this, but it is not obvious from the proofs and coding theory … Continue reading →

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Tuesday 07/09/13

Posted on July 9, 2013 by admin

9:30 A – 7:00 P, 8:15 P – 11:00 P chromatin got 10 kb segments to run. Still lots of crashes, some substantial regions lack probes if we’re fragmenting sections so we have to stitch backtogether, might as well do … Continue reading →

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Tuesday 05/4/13

Posted on May 14, 2013 by admin

10:00 A – 5:00 P, 9:00 P — 11:45 P Analysis of signal and background in different hybe conditions Discussion with Brian on temperature results Additional notes not yet sent: Hybe conditions that reduce binding to reduce background seem to … Continue reading →

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Failures update

Posted on March 1, 2013 by admin

Fly Genetics Failed knockdown: 2x Mat alpha tub driving any of the TRiP valium 20 or valium 22 PcG target shRNAs. Gives viable F1 and F2s or infertile F2s. No PcG phenotypes. Kill all current crosses Kill all expanding Gal4 … Continue reading →

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Protected: probe design

Posted on February 27, 2013 by admin

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Testing small oligopaint probes

Posted on November 10, 2012 by admin

First small test probe Reagents The primer  has a 5′ TYE665 dye. Both primers are at 200 µM (200 pmoles/µl).  template was at 10 ng/µl. A look at the genomic region Zoom in on region   Question for Ting /Brian … Continue reading →

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Protected: Experiments to try later

Posted on October 18, 2012 by admin

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observations on Insulator organization

Posted on October 16, 2012 by admin

CTCF sites are TF rich: (en locus) CTCF sites are TF rich ~HOT DNAs?  (though not as strong DNA-hypersensitive regions).  rich ~HOT DNAs?  (though not as strong DNA-hypersensitive regions). Do these insulators co-localize (most insulators HiC/3C together) Coerces 2012 MolCell chromatin domain boundaries … Continue reading →

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Protected: polycomb silencing super-resolution images at ANT-C, BX-C and En loci

Posted on October 10, 2012 by admin

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Protected: PcG in mitosis summary

Posted on February 15, 2012 by admin

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