Tag Archives: confocal

Wednesday 07/06/11

Posted on July 6, 2011 by admin

9:30 A – 4:00P Finish ON confocal of MP06Hz, 10 embryos completed (scan started at 8P) Update data on MP10_sna18, run v3 through analysis.  Changing threshold indeed does not substantially alter counts.  keep v4. Working on sna plotting.  Plotting distributions … Continue reading →

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Tuesday 07/05/11

Posted on July 5, 2011 by admin

9:50 A — 7:00 P, 7:40 P – 8:15P Research planning from lab meeting discussion. Literature browsing, nothing striking. Need repeat lab meeting on Friday Collating snail data in preliminary figures Order new flies: Dp(1;Y)BSC172, y[+] P{w[+mW.Scer\FRT.hs3]=3′.RS5+3.3′}BSC9, B[S]/winscy (brk duplication … Continue reading →

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Saturday 07/02/11

Posted on July 2, 2011 by admin

11:20 A – 11:45 A Finish ON confocal of 2.8 Hz embryos (after having lost all positions on accidental clear).

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Wednesday 06/29/11

Posted on June 29, 2011 by admin

9:40 A –3:30 P, 7:50 P – 9:20 P Finish ON confocal MP07Hz_snaD image 2.8Hz_snaD rescue for counting.  — still drift issue during acquisition as lens heats. Literature review: Set1 not Trx is major H3K4 trimethyltransferase (Lis) Trans-activation in mammalian … Continue reading →

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Wednesday 06/22/11

Posted on June 22, 2011 by admin

9:40 A -3:30 p Finish ON confocal.  Data acquisition looks good, 10 embryos finished. Rerunning unsupervised dot counting with higher threshold to reduce noise in y channel on MP09_c.  (matched .05 filter rather than .02). Layout for lab-meeting.

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Monday 06/20/11

Posted on June 20, 2011 by admin

10:00 A — 7:00P, 8:00P – 10:00P Mount slides Confocal, MP07 hets Test samples (s02, some background, weak en in test sample, need more imaging) Biophysics webpage development: added academics page and random image widget. Analyzing sna[18] MP10 data Reading … Continue reading →

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Saturday 06/18/11

Posted on June 18, 2011 by admin

2:30P – 7:00 P, 8:00P – 10:30 P in situs day 2 Analyze s07_MP08Hz data Finish confocal MP09Hz_c write response for Stas to stats paper

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Friday 06/17/11

Posted on June 17, 2011 by admin

10:00 A — 7:40P, 8:10P — 10:00P Send response letters to PIs Plan out next round of in situs in situs day 1 renamed Enhancer_modeling project to mRNA_counting as more appropriate description.  Fixed most-all links in m-code.  Still need to … Continue reading →

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Planning for chromatin condensation imaging

Posted on June 17, 2011 by admin

Planning chromatin staining inv expression pattern: maternal, broad, late seg-polarity gene. en expression pattern: early seg-polarity gene, front on first tou expression pattern.  Maternal and ubiquitous.  Yay. E(Pc) expression pattern.  ‘faint ubiquitous’.  Hope this works. 5′ probe from tou may be … Continue reading →

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Thursday 06/16/11

Posted on June 16, 2011 by admin

10:00 A – 5:00P, 11:30P – 12:30 A Finish confocal run MP06 (from slide 5-22) figures and slides for ML on hb smFISH Analyze MP01_b and MP02_b (5-22) data (first pass). drafting response letters to possible postdoc PIs

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