Tag Archives: cell labeling

Thursday 10/10/13

Posted on October 10, 2013 by admin

10:00a – 8:30p, 10:00p – 12:00a Goals finish abstract for Alvaro work on slides for Stowers STORM with Bogdan finish F1, start coverslip 4 STORM data processing with Bogdan Oligo Prep G3-G5 P1A405, P2Cy3, P4Cy3 samples Hybridizations for next small … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Thursday 10/10/13

Thursday 08/22/13

Posted on August 22, 2013 by admin

10:10a – 7:00p, 8:50p – 12:10a STORM & staining BX-C tiles AbdA labeled coverslip (S2 cells) found cross-hatching imaging additional cells record chromatic beads will restain AbdA-stained coverslip with BX-C Ubx labeled coverslip (S2 cells) found cross-hatch (much more evident … Continue reading →

Posted in Summaries | Tagged , , , , | Comments Off on Thursday 08/22/13

Tuesday 06/04/13

Posted on June 4, 2013 by admin

10:00 A – 12:30 A cell staining heating wash buffers to 47C imaged 20 bp single probe cells (5 cells). imaged 40 bp array (with 0 gain, oops). (6 cells) imaged 40 bp array for restain (7 cells) Doory imaging … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Tuesday 06/04/13

Tuesday 05/21/13

Posted on May 21, 2013 by admin

8:30 A – 5:00 P, 7:30 P – 11:45 P Probe making ethanol precipitations Ethanol precipitate B/Y/K/G probes from 2-5 mL scale synthesis (which had poor nicking and extra-large bands). Ethanol precipitate new 4 mL scale B/Y/K/U PCRs with primer-fixed … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Tuesday 05/21/13

Friday 03/29/13

Posted on March 29, 2013 by admin

9:30 A – 8:10 P, 9:00 P – 12:10 A Install cryo-attachment be sure to retract arm and center FC mount prior to mounting be sure there is space between the arm and the chamber so the arm can move … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Friday 03/29/13

Tuesday 01/29/13

Posted on January 29, 2013 by admin

10:00 A – 7:20P, 8:45P – 11:45P General As of Today, all IDT oligo orders are to go through Matt, not through HHMI card on file. Cell labeling heating up 2X SSCT wash to 60C in large hybe oven check … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Tuesday 01/29/13

Monday 01/28/13

Posted on January 28, 2013 by admin

9:45A – 10:00 P Lab meeting (notes) Fly work scored 5 more back-cross lines (no rescues still):  [spreadsheet 0AjSqkxgziU1YdENuMzFjejM0TnNXUEQ1OWdHRW1OY1E 602 202 sheet = 1] Flipped and watered bottle cross of Df/CyO,esc[2] x Sp/esc[6] males. New bottle cross of Df/CyO,esc[2] x Sp/esc[6] males … Continue reading →

Posted in Fly Work, Summaries | Tagged , , | Comments Off on Monday 01/28/13

Monday 01/14/13

Posted on January 14, 2013 by admin

9:30 A – 7:30P snail imaging copy data from confocal (55 GB file).  Run O/N at speed 7 using GASP detector projecting data from false start.  Delete lsm file (mostly blank, and the critical data has been copied into a … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Monday 01/14/13

Sunday 01/13/13

Posted on January 13, 2013 by admin

3:30P – 11:55 P Fly work Flipped most of rescue crosses.   Several vials extinct.  Should still have more vials than I want to screen though. New crosses of SCM males to MTD virgins (2 vials). Examine current crosses of … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Sunday 01/13/13

Wednesday 01/09/13

Posted on January 9, 2013 by admin

10:00A — 10:45P Cell culture blocking IF first.   secondaries for IF first (2 m-750 (6:1), 6 wells m-488 (2.0)) 10 min in .1% GA, 4% PFA post fix.  wash in PBT, then 2xSSCT following Brian’s protocol.  Need rubber cement. … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Wednesday 01/09/13