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Tag Archives: cell labeling
Thursday 10/10/13
10:00a – 8:30p, 10:00p – 12:00a Goals finish abstract for Alvaro work on slides for Stowers STORM with Bogdan finish F1, start coverslip 4 STORM data processing with Bogdan Oligo Prep G3-G5 P1A405, P2Cy3, P4Cy3 samples Hybridizations for next small … Continue reading
Posted in Summaries
Tagged cell labeling, Library2, presentation, STORM
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Thursday 08/22/13
10:10a – 7:00p, 8:50p – 12:10a STORM & staining BX-C tiles AbdA labeled coverslip (S2 cells) found cross-hatching imaging additional cells record chromatic beads will restain AbdA-stained coverslip with BX-C Ubx labeled coverslip (S2 cells) found cross-hatch (much more evident … Continue reading
Posted in Summaries
Tagged cell culture, cell labeling, images, STORM, STORM analysis
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Tuesday 06/04/13
10:00 A – 12:30 A cell staining heating wash buffers to 47C imaged 20 bp single probe cells (5 cells). imaged 40 bp array (with 0 gain, oops). (6 cells) imaged 40 bp array for restain (7 cells) Doory imaging … Continue reading
Posted in Summaries
Tagged cell labeling, embryo labeling, meetings, presentation
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Tuesday 05/21/13
8:30 A – 5:00 P, 7:30 P – 11:45 P Probe making ethanol precipitations Ethanol precipitate B/Y/K/G probes from 2-5 mL scale synthesis (which had poor nicking and extra-large bands). Ethanol precipitate new 4 mL scale B/Y/K/U PCRs with primer-fixed … Continue reading
Posted in Summaries
Tagged cell labeling, images, probe making, STORM
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Friday 03/29/13
9:30 A – 8:10 P, 9:00 P – 12:10 A Install cryo-attachment be sure to retract arm and center FC mount prior to mounting be sure there is space between the arm and the chamber so the arm can move … Continue reading
Tuesday 01/29/13
10:00 A – 7:20P, 8:45P – 11:45P General As of Today, all IDT oligo orders are to go through Matt, not through HHMI card on file. Cell labeling heating up 2X SSCT wash to 60C in large hybe oven check … Continue reading
Monday 01/28/13
9:45A – 10:00 P Lab meeting (notes) Fly work scored 5 more back-cross lines (no rescues still): [spreadsheet 0AjSqkxgziU1YdENuMzFjejM0TnNXUEQ1OWdHRW1OY1E 602 202 sheet = 1] Flipped and watered bottle cross of Df/CyO,esc[2] x Sp/esc[6] males. New bottle cross of Df/CyO,esc[2] x Sp/esc[6] males … Continue reading
Posted in Fly Work, Summaries
Tagged cell labeling, coding, fly work
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Monday 01/14/13
9:30 A – 7:30P snail imaging copy data from confocal (55 GB file). Run O/N at speed 7 using GASP detector projecting data from false start. Delete lsm file (mostly blank, and the critical data has been copied into a … Continue reading
Sunday 01/13/13
3:30P – 11:55 P Fly work Flipped most of rescue crosses. Several vials extinct. Should still have more vials than I want to screen though. New crosses of SCM males to MTD virgins (2 vials). Examine current crosses of … Continue reading
Wednesday 01/09/13
10:00A — 10:45P Cell culture blocking IF first. secondaries for IF first (2 m-750 (6:1), 6 wells m-488 (2.0)) 10 min in .1% GA, 4% PFA post fix. wash in PBT, then 2xSSCT following Brian’s protocol. Need rubber cement. … Continue reading