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Tag Archives: chromatin
Friday 03/28/14
9:45a – 11:50p Goals Record bead fields. check isoforms differences and consequences for probes selected (do our probes still hit the most popular isoform of imr90s?) Cell Staining washout stains for new multi-color samples. Need to wait until oven is … Continue reading
Thursday 04/27/14
10:15a – 12:30a Goals Data processing copy C05 and C06 data to hard-drives Launch analysis of C03-C06 data plate, fix, prep and hybe new cells Probe making RT reactions test gel probe purfication cell staining wash out probes image multi-color … Continue reading
Posted in Chromatin
Tagged cell staining, chromatin, images, probe making, STORM
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Wednesday 03/26/14
9:20a – 10:10p, remotely, 10:50p – 11:30p Goals passage cells washout probe from new multicolor stain finish updating notes from yesterday STORM run configurations data analysis launched break down PRISM2 run of L3C04. (complete) break down STORM2 run of L3C03 … Continue reading
Tuesday 03/25/14
10:00a – 1:00a Oligo Secondaries Fixes for secondary figure use the dots only rendering method make dots only rendering method a standard plugin for matlab-storm (I rather like it) wrote function StormHist.m added to QuickPlots of matlab-functions instead, not sure … Continue reading
Monday 03/24/14
10:00a – 11:15p Chromatin Project Data summaries making slides to summarize recent analysis of multi-color data see slides for 3/28/14 XZ meeting Data analysis F03-F04 data chromewarps obviously don’t match data, clear multi-pixel shift up and to the right on … Continue reading
git remove binary files
See current file size. First need to git gc Alistair@MONET ~/Documents/Research/Projects/Chromatin/Code (master) $ git gc Counting objects: 713, done. Delta compression using up to 8 threads. Compressing objects: 100% (570/570), done. Writing objects: 100% (713/713), done. Total 713 (delta 257), … Continue reading
Posted in Software Development
Tagged chromatin, git, troubleshooting
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Friday 03/21/14
10:30a PH project results at greater sampling depth, longer simulation time, for the current published table parameters see PH project version corresponding to current date for details. Data\2014-03-17_PhSim\PhCell_CappingModel5.mat Research Organization Reorganizing projects folder Freezer space organization started new box: Lib3 … Continue reading
Thursday 03/20/14
10:15a – 12:15a Project 2 see protected notes Chromatin Project Probe Making RTs 4 uL primer gel looks good, basically complete incorporation. Maybe try 8 uL primer next time? (going to need to order more primer!) probes in 4C walkin, … Continue reading
Monday 03/17/14
9:45a – 8:00p Computer Maintenance security updates on Cajal + reboot security updates on Tuck – reboot postponed due to users still analyzing data write to CDW about is this software or just a number: 2012 server why 2 versions … Continue reading
Sunday 03/16/14
10:30a – 6:00p Goals flip fly stocks (done) passage cells (done) scheduling christia (May 17-20) dad’s conference (April 25th) dentist (May 7th) More data processing! running splitdax on 140310_L4E4 data running bead averaging on 140310_L4E4 data analyzing averaged beads from … Continue reading