Tag Archives: embryo labeling

Sunday 10/28/12

Posted on October 28, 2012 by admin

10:30 A – 1:45P, 4:45 – 8:15P Fly Work 10:30A collect virgin Espl/sim 12:00 P, flip collection cages 7:30 P, fix embryos, 0.5 – 7 hrs MTDs, Psc 1, and Pc 3.  Left on shaker.  Good embryos should be sunk … Continue reading →

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Saturday 10/27/12

Posted on October 27, 2012 by admin

10:30A – 8:ooP Stop O/N confocal by 11A. Confocal data check: Late positions start too low.  Not sure why — maybe middle position to close to edge changed microscope positions? Fly Work Collect virgin Espl[D]/ sim[D].   Flip fly stocks … Continue reading →

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Wednesday 10/24/12

Posted on October 24, 2012 by admin

9:15 A – 7:00P, 8:00P – 12:00A RNA FISH + Immuno-stain in cultured cells (developing protocol:) warming up hybe solution for hot washes remove, label and save probes (in yellow -20C dilute probes box, labeled as S2). 3x Hot wash … Continue reading →

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Tuesday 10/23/12

Posted on October 23, 2012 by admin

9:20 A – 6:30P, 8:00P-11:00P (remote) S2 cell DNA-FISH labeling Fixing S2 cells for DFISH (1/2x PBT 5% formaldehyde) cells still attached, looks promising. cells preincubated at 92C for 3min, 60C for 20min, moved to RT probes denatured at 92C … Continue reading →

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Monday 10/22/12

Posted on October 22, 2012 by admin

9:20 A – 8:30, 9:30 – 11:10PM Flip collection cage take embryos out of 4C, final hour incubating at RT before washes. snail in situs Draq5 stained and mounted STORM / section in situs / DFISH all in secondaries at … Continue reading →

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Sunday 10/21/12

Posted on October 21, 2012 by admin

11:00 A-3:00P, 7:00P – 9:40 PM upgrade linux laptop to 12.10 Review writing reading /re-reading recent literature White/Barolo/Cohen paper He / Sinha model see post Experiments Rinse out probes from DNA FISH (probes saved – green box) Rinse out probes … Continue reading →

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Sunday 10/14/12

Posted on October 14, 2012 by admin

10:20 A Fly work New cage of MTD (not crossed, just as control).  Use for Pc and Su(Z)12 staining.  Methanol treat after primaries to improve in situ. DNA FISH Day 3: DNA-FISH + antibody. [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE sheet=8 602 302] postfix 1 … Continue reading →

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Saturday 10/13/12

Posted on October 13, 2012 by admin

11:00A – 6:ooP, 9:ooP-11:00P binomial statistics and confidence intervals discussion with JM Collect virgin E(spl) and sim[D] flies, 4 more vials of crosses. Finished virgin collections and cross-setups for present.  All flies now on RT shelf.  Organizing Matlab STORM library … Continue reading →

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Friday 10/12/12

Posted on October 12, 2012 by admin

9:45 A – 7:00 P, 8:00P-9:15P Fly work collect virgins: E(spl)[D], sim[D], MTD new bottle cross: MTD virgins x male TRiP Scm shRNA 5 new vials cross E(spl)[D] x sim[D] collect PM virgins E(spl) and sim MTD moved back to … Continue reading →

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Wednesday 08/01/2012

Posted on August 1, 2012 by admin

10:10 A – 7:30P, 8:20P – 10:45P Flip collection cages. Meeting with Ting and Xiaowei. KE miniprep cg5… cg10… and bxd samples.  lab colonies did not grow in liquid culture?  Sent samples for sequencing. STORM of Pc6_cntrl Label new dk-rb-750 … Continue reading →

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