Tag Archives: fly work

Wednesday 01/23/13

Posted on January 23, 2013 by admin

10:30 A – 8:30P, 9:30P – 11:45P (8:45-10:30A workout) Fly Work collect virgins: Df/CyO,esc[2],  2x mat-tub Gal4. collect males: esc[6]/Sp. cross (1 new bottle) esc[6]/Sp x Df/CyO,esc[2] (should have used Gla, will need to screen Sp for all flies that … Continue reading →

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Tuesday 01/22/12

Posted on January 22, 2013 by admin

  Fly Work collect virgins (df/CyO-Esc[2])  AM # New crosses with remaining MTDs! # collect virgins PM Ultra-cryo sectioning yw embryos Cutting sample at -51C, knife at -54C, TG -50C at .65 – .75 um cuts pretty well, still better … Continue reading →

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Wednesday 01/16/13

Posted on January 16, 2013 by admin

9:30 A – 7:30 P  Finish comments on counting comparison for ML # Finish revisions Write to MERCK about Probe Making Precipitate and resuspend RNA-FISH DNP probes for tou, inv, en, Antp and Dfd. Make new RNA-Hybe soln to resuspend … Continue reading →

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Wednesday 01/09/13

Posted on January 9, 2013 by admin

10:00A — 10:45P Cell culture blocking IF first.   secondaries for IF first (2 m-750 (6:1), 6 wells m-488 (2.0)) 10 min in .1% GA, 4% PFA post fix.  wash in PBT, then 2xSSCT following Brian’s protocol.  Need rubber cement. … Continue reading →

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Tuesday 01/08/13

Posted on January 8, 2013 by admin

10:00A – 10:30P (8:30-10A lab exercise/training) Bistability First draft of deterministic model figure and corresponding text Trying to figure out correct integral to compute an energy surface from the vector field.  Not working so far. Fly work collect virgins: (now … Continue reading →

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Monday 01/07/13

Posted on January 7, 2013 by admin

9:30 A – 8:30P, Probe making Emulsion PCR of engrailed region probe at Wu lab.   Final library concentration 16 ng/uL, in 14 uL.  Should PCR amplify once. Brian will run test PCR and test gel to check library quality … Continue reading →

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Sunday 01/06/12

Posted on January 6, 2013 by admin

11:00 A – 10:30 P snail analysis Finish O/N confocal of MP06Hz Finish O/N confocal of MP08Hz sna[D] extract images from lsm, analyze nuclear density and record staging run dot-finder to count mRNA MP08Hz conclusions:  2 def. mutants in mid … Continue reading →

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Friday 01/04/12

Posted on January 4, 2013 by admin

7:00P – 9:30P Fly work clear for virgin collection tomorrow: DB Sp/CyoZ; TM2/TM6 MTD sna[18]/CyoZ/+; TM3/+/Pr esc[6]/CyO Df/CyO,esc[2] E(spl)[D] sim[D]

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Monday 12/17/12

Posted on December 17, 2012 by admin

9:40 A – 8:00P, 9:30-10:30P final prep for lab meeting Give lab meeting presentation 10A-1P Meeting with XZ, ~4P Meeting with Max, discuss MTD knockdown ~4P Snail Missing JPA and highlights for CR paper, upload now Cell reports submission site … Continue reading →

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Tuesday 12/11/12

Posted on December 11, 2012 by admin

10:10 A Check PCRs (in blocks 3A (sim screening) and 3B (Espl screening) better way to do this: complementation test back to original sim[D] and Espl[D] lines.  Just need to expand those lines back to bottles, will need ~100 vials … Continue reading →

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