Tag Archives: mRNA counting

Protected: MP06b (cc13 no shadow)

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hb y Hz data summaries

Set missG to 1 for all data sets. Using raw density instead of size-adjusted counts per cell subtracting maternal contribution post processing as removing lowest cell count. Without maternal correction: 

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Friday 07/08/2011

8:30 A – 6:00 P ON confocal failed, too much drift. Organizing hb data into lab meeting part 2. writing embryo matching script using wildtype profiles for hb Rewrote anlz_hb_gradient_data.m and Plot_hb_data.m to rely just on raw density rather counts … Continue reading

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Thursday 07/07/2011

8:30 A — 4:50 P working on Plot_hb_data: match embryos based on endogenous gradients Reprocessing MP09 data, the .05 for speed on wt is a little too high, causes some dot loss, drop to a more conservative .03 or .01. … Continue reading

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Protected: MP05Hz analysis

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Wednesday 07/06/11

9:30 A – 4:00P Finish ON confocal of MP06Hz, 10 embryos completed (scan started at 8P) Update data on MP10_sna18, run v3 through analysis.  Changing threshold indeed does not substantially alter counts.  keep v4. Working on sna plotting.  Plotting distributions … Continue reading

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Protected: snail rescue

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Protected: sna 2.8 enhancer rescue data

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Protected: New conclusions from MP08 and MP07 rescue data

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Protected: cntrl-y BAC in sna point mutant null

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