Author Archives: admin

Protected: project 2

Posted on June 3, 2013 by admin

There is no excerpt because this is a protected post.

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Monday, 06/03/13

Posted on June 3, 2013 by admin

10:00A – 7:00P, 8:00P-10:00P Lab meeting see notes STORM attempted TCEP 750 imaging (failed last night due to un-reproducible error with quadview) 750 is bright now but very few switching dyes. loads of brightly switching Cy5 dyes suggest that buffer … Continue reading →

Posted in Summaries | Comments Off on Monday, 06/03/13

Protected: lab meeting 06/03/13

Posted on June 3, 2013 by admin

There is no excerpt because this is a protected post.

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Sunday 06/02/13

Posted on June 2, 2013 by admin

10:00 A – 8:00 P Ph-FLAG analysis / clustering Blocking total counts per cell (after applying mask) results in good comparison. Currently 50-300K: window probably a bit large but need to get the cell numbers up. Ph wt data: Buffer … Continue reading →

Posted in Summaries | Comments Off on Sunday 06/02/13

Saturday 06/01/13

Posted on June 1, 2013 by admin

10:00 A – 12:15 P, 3:00 P – 7:00 P STORM finish O/N STORM run. VALAP seal did not work as well this time — substantial air bubble in sample, switching not over airbubble but background is up and signal … Continue reading →

Posted in Summaries | Comments Off on Saturday 06/01/13

Friday 05/31/13

Posted on June 1, 2013 by admin

10:00 A – 7:00P, 9:30P – 12:30 A Check RNA hybes on STORM2 (hybed at 50C (55C?) in dry oven O/N in coverglass preps like for DNA labeling) (quick check on turnkey looked promising) tub looks good but hard to … Continue reading →

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Protected: project 2

Posted on June 1, 2013 by admin

There is no excerpt because this is a protected post.

Posted in Research Planning | Comments Off on Protected: project 2

Thursday 05/30/13

Posted on May 30, 2013 by admin

10:00 A – 7:00P, 8:30P – 12:40A File maintenance Alistair12 out of disk space! Black-DNA region run crashed due to running out of disk space STORM analysis crashed due to no free disk space to save files STORM analysis Fab7 … Continue reading →

Posted in Summaries | Comments Off on Thursday 05/30/13

Wednesday 05/29/13

Posted on May 29, 2013 by admin

8:00 PM – 11:20 P probe making Set up new test gel: 3R4500, Ubx, AdbdA, AbdB, BX-C run OPAINT, and OPAINT1 72C cell staining plate new Kc167 cells on coverslips Fix run through all prep steps up to 50% formamide … Continue reading →

Posted in Summaries | Comments Off on Wednesday 05/29/13

Tuesday 05/28/13

Posted on May 28, 2013 by admin

10:10 A – 5:30 P RNA labeling of cultured cells incubate cells in 55C RNA hybe buffer, 30 min transfer to RT 2x SSC, wash 10 min transfer to PBT wash 10 min+ 2x block 1 hour stain with shp … Continue reading →

Posted in Summaries | Comments Off on Tuesday 05/28/13