Author Archives: admin

Protected: Ph analysis 2

Posted on May 27, 2013 by admin

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Monday 05/27/13

Posted on May 27, 2013 by admin

11:00 A – 10:00 P Probe and hybe troubleshooting check staining AATAT control and Y look good black maybe stained, check after washout low and high RNA-en-P1 both have substantial nuclear background, but no clear signal Coding Fixed bugs in … Continue reading →

Posted in Summaries | Comments Off on Monday 05/27/13

Sunday 05/26/13

Posted on May 26, 2013 by admin

11:00 A – 7:30 P, 9:00 P – 11:00 P Trouble-shooting hybes check samples: AATAT-P1 + S1-short on cells – strong and clear en-P1 + S1-short on cells – clear staining, bleaches fast en-RNA-P1rc + S1-short on cells – no … Continue reading →

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Saturday 05/25/13

Posted on May 25, 2013 by admin

10:20 A -7:30 P Project 2 Piranose: staining is still good in piranose treated cells. GA background is EXTREME, but certainly uniform, not punctate on the whole cell conventional level at least Cell staining check cells from yesterday — all … Continue reading →

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Friday 05/24/13

Posted on May 24, 2013 by admin

10:30 A – 8:35 P Probe making resuspend ethanol precipitated probe samples make and cast new UREA gels. 2x 15 well .75mm, 1x 5 well 1.5 mm (25 mL is not enough for 2x 1.5mm — these take 10mL each. … Continue reading →

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Thursday 05/23/13

Posted on May 23, 2013 by admin

11:10 A – 7:30 P, 9:30P – 10:30 P probe making Labeling amine-tagged probe with cy5 dye. labeling reaction for secondary cy5 (see protocol) labeled precipitated DNA pellet awaiting HPLC purification (need directions from Hao). Test large scale reactions with … Continue reading →

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Wednesday 05/22/13

Posted on May 22, 2013 by admin

10:20 A – 7:15 P, 8:30 P – 1:45 A cell staining remove samples from 40C, place in RT 2x SSCT for first rinse out. check staining of control slide on STORM2 TIRF- no staining except in dead cells (should … Continue reading →

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Tuesday 05/21/13

Posted on May 21, 2013 by admin

8:30 A – 5:00 P, 7:30 P – 11:45 P Probe making ethanol precipitations Ethanol precipitate B/Y/K/G probes from 2-5 mL scale synthesis (which had poor nicking and extra-large bands). Ethanol precipitate new 4 mL scale B/Y/K/U PCRs with primer-fixed … Continue reading →

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Protected: Project 2

Posted on May 21, 2013 by admin

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Posted in Research Planning | Comments Off on Protected: Project 2

Monday 05/20/13

Posted on May 20, 2013 by admin

9:20 A – 7:00P, 9:30P — 12:20 A Lab meeting visiting post-doc candidate: notes Probe making figure out increased library concentrations for large scale PCR for B/Y/K/G libraries set up 5 mL scale PCR for B/Y/K/G using nick-containing primers Setup … Continue reading →

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