Author Archives: admin

Sunday 05/12/13

Posted on May 12, 2013 by admin

10:00 A — 8:00 P, 9:15 P – 11:30 P OligoPaint Probes new PCR Pool samples: 4 mL Ubx, AbdA and AbdB, 2 mL each B, Y, K and G. cooling down ultra-centrifuge (takes 3 hours, good thing to start … Continue reading →

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Saturday 05/11/13

Posted on May 11, 2013 by admin

3:00 P – 8:00 P, 9:30 P – 11:15 P Kinetic modeling Discussion with Jeff sent text books (see email) Lent library book (Feller) to Jeff should write text together? OligoPaint Probe making spin down ethanol precipitating samples of probe … Continue reading →

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Friday 05/10/13

Posted on May 10, 2013 by admin

9:00 A – 10:33 P OligoPaint Probe making Spin down crushed gels and aspirate fluid. Attempt to nanodrop. ~200/UV peak, readings probably meaningless. Could Qubit but hopefully these are single-stranded now and shouldn’t read. nanodrop no 260 peak, so reading … Continue reading →

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Protected: project 2 update

Posted on May 10, 2013 by admin

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Thursday 05/09/13

Posted on May 9, 2013 by admin

9:30 A – 7:30 P, 8:30 P – 12:30 A Literature Interesting paper by Sanchez and Gore on altruists, cheating, and ecological stability in yeast Clear neat article in Nature on being a scientist. Oligo Paint probes Pool O/N PCRs … Continue reading →

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Wednesday 05/08/13

Posted on May 8, 2013 by admin

9:30 A — Probe making pool digests aliquot out test DNA: 1 uL digest DNA, 3 uL TBE, 1 uL 6x loading dye + 12x gel green, 5 uL formamide with 5% 5M EDTA Pour new 16% urea PAGE gels … Continue reading →

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Protected: Ph-Flag notes

Posted on May 7, 2013 by admin

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Tuesday 05/07/13

Posted on May 7, 2013 by admin

9:25 A -5:50 P Ph FLAG Processing confocal images of Ph data updated ImageShop / ExtractLSM to fix bug in multi-position loader. Ph FLAG discussion / comments: post Literature Pirrotta Insulators target active genes to transcription factories and polycomb-repressed genes … Continue reading →

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nanodrop

Posted on May 7, 2013 by admin

measurements (ng/uL). in 150 uL unless otherwise indicated Y2.5 E1 263 Y2.5 E2 81 Y1.5 E1&2 272 K2.5 E1 519 K2.5 E2 435 K2 E1 150 K2 E2 100 K2 E3 48 (2x + reblank. prev 16?!) G E1 97 … Continue reading →

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Protected: Ph FLAG and Ph FLAG M

Posted on May 7, 2013 by admin

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