Author Archives: admin

Protected: project 2 notes

Posted on April 17, 2013 by admin

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qubit measurements

Posted on April 17, 2013 by admin

All numbers in ng/uL (equivelantly ug/mL). Original volume is 1 mL so this equivelantly DNA mass. Though some samples were split multiple ways, EtOH is 2:1 with vs without glycogen. Filtration was done with 150 uL of 1 mL PCR. … Continue reading →

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Protected: project 2 coding

Posted on April 16, 2013 by admin

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Tuesday 04/16/13

Posted on April 16, 2013 by admin

9:30 A – 8:00 P, 9:00P – 12:00 A OligoPaints Set up EtOH precipitation of samples 1-5. In -20C, ~10:00 A. Pour 5% agarose Na-Borax gel to try to diagnose issue with ladder: not all bands showing up on urea … Continue reading →

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Monday 04/15/13

Posted on April 15, 2013 by admin

9:30 A – 8:00 P, 9:00 P – 11:55 P Meetings group meeting (notes) journal club (notes) discussion with Xiaowei (notes) Gels At least 3 ways to screw up gels APS really needs to fresh, (try 140 uL instead of … Continue reading →

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Protected: new project 2

Posted on April 15, 2013 by admin

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Structural and molecular interrogation of intact biological systems

Posted on April 15, 2013 by admin

CLARITY hydrogel electrophoretic tissue transmutation. Nature Paper cross link gel. Add ionic detergent (SDS micelle), extract all membranes in electrophoresis tissue becomes much clearer, can image deep proteins still there. do they demonstrate nucleic acids? – doesn’t look like it. … Continue reading →

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Protected: lab meeting 04/15/13

Posted on April 15, 2013 by admin

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Sunday 04/14/13

Posted on April 14, 2013 by admin

5:30 P – 8:00P OligoPaint Analyzing previous gel images Conclusion: something went weird, possibly with original PCR used to seed most of the second round PCRs. Plan Repeat PCRs from scratch Run diagnostic denaturing gels at each step in addition … Continue reading →

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experimental oligopaint PCR 3

Posted on April 14, 2013 by admin

Samples (1 mL scale) y nicked 2x Ya-primer b nicked 1x Ba-primer + 1 x CPPT ubx nicked 1x UBx-adapter-primer + 1x CPPT y PPT: 5 uL YA + 20 uL PPT y PPT control: 5 uL YA + 20 … Continue reading →

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