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Author Archives: admin
Wednesday 12/04/13
9:40a – 11:30p Goals show Hao new project 2 results RNase-Away / bleach treat everything on my bench fix and stain new RNA cells. Allow 1 hour prehybe. Project 2 See notes Chromatin colors, double stain prep Probe organization reorganized … Continue reading
Posted in Summaries
Tagged cell labeling, images, probe making, project2, STORM
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Tuesday 12/03/13
9:15a – 11:15p Goals Call Dad, happy birthday Analyze Ph polymer simulations for meeting with Ting. STORM Finish D07 on STORM2 (9 kb green, transcribed, RNaseA treated). Looks transcribed – variable spots, larger than expected for only 9 kb maybe? … Continue reading
Monday 12/02/13
9:00a – 12:20a Goals work on data analysis / slides for meeting with Ting tomorrow new round of yellow chromatin RNA DNA double stains. Include attempt restain E10rna. Still needs RNase. 3D z-calibration [POSTPONED] Start statistical analysis of Blue Polymer … Continue reading
Sunday 12/01/13
9:30a – 7:45p, 10:00p – 12:15a Genomics Cajal slowing down (2536 of 4385. Though all 4 variants of stringency is 4x 4385) CPU cap semi-respected, code is waiting for free cpu dips to launch new jobs. Target is 75%. Running … Continue reading
Protected: Saturday 11/30/13
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Posted in Summaries
Tagged cell labeling, coding, STORM, zcal
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Protected: Friday 11/29/13
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Posted in Summaries
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Protected: Thursday 11/28/13
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Wednesday 11/27/13
Goals Sequencing analysis plots of coverage uniformity per probe set plots of failed probe vs. purity using new indexed method (or maybe just a length cut-off) Ph Project restart simulations Genomics start building library 4 (on Monet) start building library … Continue reading
Tuesday 11/26/13
9:15a -10:00p Goals Restart Fasta file building for genomics Coding removing GPU from STORMrender not compatible with CUDA 5.5 not restorable after CUDA update not distributable / not multiplatform Genomics Computer reboot terminated building gene list, around 60% complete Relaunched … Continue reading
Posted in Summaries
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Monday 11/25/13
9:10a – 11:45p Meetings Lab Meeting: private notes Journal Club: notes Mentoring Meeting Bogdan will explore length dependence in Blue domains BXC ~ 1900 nucleosomes — feasible scale for simulation Polymer simulations Bogdan attempting to get GPU accelerated openMM working … Continue reading