Author Archives: admin

Sunday 09/10/13

Posted on November 10, 2013 by admin

9:00a – 1:30p, 5:30 – 11:00p Goals Continue analysis of MiSeq Results (in process) Work on modeling figures for Ph Project Finish editing Banff Workshop report and send around for final review prior to submission. (tomorrow?) Double check requirements for … Continue reading →

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Protected: project 2 update

Posted on November 10, 2013 by admin

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Saturday 11/09/13

Posted on November 9, 2013 by admin

8:30a – 7:30p, 8:30p – 11:30p (remotely) Goals Write to Alvaro (done) Reply to Zenklusen (done) Continue analysis of MiSeq Results (in process) write to Jeff to get gene list (done) repeat RT reaction with more enzyme (done) Work on … Continue reading →

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Friday 11/08/13

Posted on November 8, 2013 by admin

10:00a – 11:45p Goals Write to Alvaro — try tomorrow Reply to Zenklusen — try tomorrow Check MiSeq Results in process repeat RT reaction with more enzyme — try tomorrow Project 2 see notes Review writeup Install phase ring and … Continue reading →

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Protected: project 2 to do

Posted on November 8, 2013 by admin

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Thursday 10/07/13

Posted on November 7, 2013 by admin

9:25a – 11:50p Probe Making very poor probe incorporation. Maybe the lower concentration of Maxima? Try to run RT again tomorrow to compare. attempted DCC of probes to remove excess primer using short probes 1-4. Extremely low yield (20 ng/uL … Continue reading →

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Wednesday 11/06/13

Posted on November 6, 2013 by admin

8:30a – 12:20a Morning tasks give Jeff deep-seq prep’d sample 4 (F3) to run as qPCR standard All analysis tasks on Cajal and all split-dax running from Monet completed. Restart Monet to install new 4Tb HDs: ProBox5 & 6. Move … Continue reading →

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Tuesday 11/05/13

Posted on November 5, 2013 by admin

10:00a – 12:30a Probe Making New short probes D07 9 kb Green transcribed E02 13 kb green (between R/Y) E03 BLUE 13 KB (no genes, good PC/Psc) E04 BLUE 10 Kb (flanked by strong PolII) E05 BLACK/blue 54 kb region … Continue reading →

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Monday 11/04/13

Posted on November 4, 2013 by admin

8:40a – 12:50a Goals Cell culture check cells. Probably all need to grow at least another day or two Show BB the contaminated cultures for teachable moment. Cell staining ~ 3-4 pm start hot washes for Y RNA vs DNA … Continue reading →

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Protected: lab meeting 11/04/13

Posted on November 4, 2013 by admin

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