Author Archives: admin

Tuesday 09/24/13

Posted on September 24, 2013 by admin

11:00a-4:30p, 8:00p-11:00p Goals: Cell culture plate cells on Poly-Lysine coverglass (with Bogdan ?) passage remaining cells (with Bogdan ?) Prep 9/2 plate and new plate for DNA FISH. Staining O/N primary staining of Pc and anti-flag. Probe making RNA cleanup … Continue reading →

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Monday 09/23/13

Posted on September 23, 2013 by admin

9:30a – 7:30p, 9:00p – 11:50p Meetings 10a, lab meeting 11:45a, meeting with Ting and Fred Need to send letter to team. Cell in situs Wash out primary probes STORM E6 (tertiary approach still) — very weak spots visible in … Continue reading →

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Protected: lab meeting 09/23/13

Posted on September 23, 2013 by admin

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Sunday 09/22/13

Posted on September 22, 2013 by admin

5:30p – 8:30p Bogdan / Rotation project prep: move black IVT reactions to top of upstairs -20C freezer new DNA FISH in situs E6 (E(Pc) region, 15kb) F6 (Y/R 140 kb) Stain E6/F6 originals with 5 uL + 1 uL … Continue reading →

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Notes for deep sequences of libraries

Posted on September 22, 2013 by admin

Bauer Core equipment training option 1 Amplify sublibraries * Buy NEB DNA kit, end repair * blunt end ligation alternative PCR primer gel-extraction Qubit concentration (nanodrop?) [optional] BioAnalyzer at Bauer Core. [optional] qPCR, dilute sample a few times. By Illumina … Continue reading →

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Friday 09/20/13

Posted on September 22, 2013 by admin

10:15a – 6:30p, 8:30p-10:00p Planning Discussion meeting with Ting 10:15a-11:15a (see notes) Planning embryo Pc next steps (see notes) Writing continue shortening BJ review Re-read and submit referee comments. Added extensive additional discussion on conclusions. Experiments with Bogdan Set up … Continue reading →

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Thursday 09/19/13

Posted on September 19, 2013 by admin

9:00a – 7:45p, 10:00p – 11:00p Writing Referee work working remotely (9a-12p) write up comments BJ review Goal: Trim down BJ review by 3 of 16 pages started trimming from beginning. 1 full page shorter. mRNA indexing human genome 22917 … Continue reading →

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Wednesday 09/18/13

Posted on September 18, 2013 by admin

9:15a – 11:30p Writing working on BIRS workshop report (9:15a- 12p) Probe making / training with Bogdan test PCR from yesterday common primer concentration was a bit low gel didn’t fully dissolve, might have affected gel running: Repeat PCRs. Repeat … Continue reading →

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Tuesday 09/17/13

Posted on September 17, 2013 by admin

Library negative controls Get 10% bleach for spray bottle. Bleach all pipettes. Get all new tips 25 uL Phusion reactions 10 uM primer dilutions Fresh library 1:100 dilution 1 uL + 1.25 uL 10uM common Fresh library 1:100 dilution 1 … Continue reading →

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Sunday 09/15/13

Posted on September 15, 2013 by admin

Shvartsman Bomyi lim (grad student) Intro Michaelis Menten / invertase. disrupted last bastion of vitalism did not know what enzyme was, could not obseve ES complex, proof was purely kinetic enzymes modyifing proteins may have less specificity — region bound … Continue reading →

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