Category Archives: Summaries

12:00 pm – 6:30 pm, working on applications Submitted all apps with Nov 1st deadlines.

9:30 am – 5:00 pm Applications working on Harvard MCB application applications won’t submit requests to letter writers until application is complete. Princeton application also won’t submit requests to letter writers given that a motivated candidate would like to have … Continue reading →

9:00 am Deep seq final prep running tape-station samplesname, conc pg/uL, index, tube Ph1, 496, 1, A M1, 442, 2, A M2, 665, 4, – 10-22 #1, 652, 1, B 10-22 #2, 516, 2, B 2W, 617, 3, B 2P, … Continue reading →

9:30 am – 7:00 pm, 9:00 pm – 11:00 pm Deep seq prep continued with deep-seq prep finished second strand synthesis ran clean up on DNA-XP beads from Bauer ran adaptor ligation. premixed adaptor and enzyme mix (2 min on … Continue reading →

8:30 pm – 11:15 pm RNA deep-seq prep sample order (8 samples): 10-22 n1, 10-22 n2, 2W, 2P, 2-4W, 2-4P, 4W, 4P running sample isolation by magnetic polyT beads, following manufacturer’s protocol. 3 samples dehydrated in last elution step. I … Continue reading →

9:00 am pairing meeting rehearse talk (8:45-9:15) see notes Experiments: QPCR for RNAi (9:15 am – 11:05 am) RNA concentrations from yesterday: 600 – 1200 ng/uL (55 uL volume) ran RNase H digestion last night, at 4C in block O/N. … Continue reading →

9:15 am – 6:30 pm presentations revising presentation for Pairing meeting with suggestions from BB (9:30 am – 12:30 pm) practice presentation Experiments 1 well of Ph KD and WT took through the RNAi treatment / wash / serum starve … Continue reading →

8:30 am – 7:00 pm background reading background research on subtelomeres for new XZ (8:30 a – 10:00 a) see notes and email. presentation prep working on polycomb bridging presentation for pairing meeting (2:30 – 7:00 pm) Imaging (10:00 am-1:00 … Continue reading →